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International Urogynecology Journal

Published in:

01-03-2012 | Original Article

The effect of raloxifene, a SERM, on extracellular matrix protein expression of pelvic fibroblasts

Authors: Jung Han Lee, Yan Wen, Mary Lake Polan, Bertha Chen

Published in: International Urogynecology Journal | Issue 3/2012

Abstract

Introduction and hypothesis

We hypothesize that the abnormal extracellular matrix (ECM) turnover in pelvic tissues of women with prolapse may be attenuated by raloxifene. We examine the effect of raloxifene on ECM protein expression in pelvic fibroblasts.

Methods

Pelvic fibroblasts were isolated from cases (N = 6) and controls (N = 3). Cells were treated with raloxifene. Dose–response analyses were performed by ANOVA. mRNA and protein expression of collagen I, III, MMPs, and TIMPs were determined by RT-PCR and Western blot. MMP activity was analyzed by zymography.

Results

The mRNA expression of TIMP-3 and protein expression of TIMP-1 and TIMP-3 were significantly increased by raloxifene in fibroblasts from both cases and controls (P < 0.05). Collagen I, III, and MMP mRNA and protein expressions were not affected.

Conclusions

Raloxifene selectively attenuates abnormal matrix degradation by increasing inhibitors of proteases, TIMPs, in pelvic fibroblasts. This opens the possibility for SERMs to be used as preventive therapy for pelvic floor disorders.

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Title: The effect of raloxifene, a SERM, on extracellular matrix protein expression of pelvic fibroblasts
Authors: Jung Han Lee
Yan Wen
Mary Lake Polan
Bertha Chen
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 3/2012
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-011-1567-0

At a glance: The STEP trials

Springer Medicine

The effect of raloxifene, a SERM, on extracellular matrix protein expression of pelvic fibroblasts

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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