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International Journal of Colorectal Disease
Published in: International Journal of Colorectal Disease 2/2019

Open Access 01-02-2019 | Original Article

The effect of pasireotide on intestinal anastomotic healing with and without whole-body irradiation in a rat model

Authors: Gabriel J. Seifert, Gunnar Leithold, Birte Kulemann, Philipp A. Holzner, Torben Glatz, Jens Hoeppner, Simon Kirste, Goran Marjanovic, Claudia Laessle

Published in: International Journal of Colorectal Disease | Issue 2/2019

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Abstract

Objective

To examine pasireotide’s effect on intestinal anastomotic healing under physiological conditions and following preoperative whole-body irradiation.

Material and methods

Forty-five male Wistar rats received an ileoileal end-to-end anastomosis. Group 1 (Co, n = 9) served as control. Group 2 (SOM, n = 10) received pasireotide (60 mg/kg) 6 days preoperatively. Group 3 (R-Co, n = 13) was subjected to 8 Gy whole-body irradiation 4 days preoperatively. Finally, group 4 (R-SOM, n = 13) received pasireotide 6 days preoperatively and whole-body irradiation 4 days preoperatively. On postoperative day 4, anastomotic bursting pressure, histology, IGF-1 staining, and collagen density were examined.

Results

Mortality was higher in irradiated animals (30.8% vs. 5.3%, p = 0.021), and anastomotic bursting pressure was significantly lower (median, R-Co = 83 mmHg; R-SOM = 101 mmHg; Co = 149.5 mmHg; SOM = 169 mmHg). Inflammation measured by leukocyte infiltration following irradiation was reduced (p = 0.023), and less collagen was observed, though this was not statistically significant. Bursting pressure did not significantly differ between Co and SOM and between R-Co and R-SOM animals respectively. Semi-quantitative scoring of IGF-1, fibroblast bridging, or collagen density did not reveal significant differences among the groups.

Conclusion

Whole-body irradiation decreases the quality of intestinal anastomotic wound healing and increases mortality. Pasireotide does not significantly lessen this detrimental effect.
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Metadata
Title
The effect of pasireotide on intestinal anastomotic healing with and without whole-body irradiation in a rat model
Authors
Gabriel J. Seifert
Gunnar Leithold
Birte Kulemann
Philipp A. Holzner
Torben Glatz
Jens Hoeppner
Simon Kirste
Goran Marjanovic
Claudia Laessle
Publication date
01-02-2019
Publisher
Springer Berlin Heidelberg
Published in
International Journal of Colorectal Disease / Issue 2/2019
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI
https://doi.org/10.1007/s00384-018-3193-5

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