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01-05-2014 | Original Article

The effect of gamma radiation on the lipid profile of irradiated red blood cells

Authors: Grazielle Aparecida Silva Maia, Cristiane de Oliveira Renó, Jorge Mansur Medina, Alan Barbosa da Silveira, Julio Alberto Mignaco, Georgia Correa Atella, Vanessa Faria Cortes, Leandro Augusto Barbosa, Hérica de Lima Santos

Published in: Annals of Hematology | Issue 5/2014

Abstract

An investigation into the effects of irradiation and of the storage time on aging and quality are a relevant issue to ensure the safety and the efficiency of irradiation in the prevention of transfusion-associated graft-versus-host disease (TA-GVHD). In this work, the biochemical properties and alterations presented by erythrocyte membranes, up to 28-days post-irradiation, with a dose of 25 Gy, were studied as a function of storage and post-irradiation time. There was a considerable variation in the total of phospholipid content, when comparing the control and irradiated samples, mostly from the third day onwards; and at the same time, the effect occurred as a function on the storage time of blood bags. The levels of total cholesterol decreased 3–9 days after irradiation. TBARS levels were increased after irradiation and 7 days of storage, but no increment of catalase activity was observed after the irradiation. Furthermore, the protein profile was maintained throughout the irradiation and storage time, until the 21st day, with the presence of a protein fragmentation band of around 28 kDa on the 28th day. In conclusion, although gamma irradiation is the main agent for the prevention of TA-GVHD, a better understanding of the physical and biochemical properties of erythrocytes are necessary to better assess their viability, and to be able to issue more secure recommendations on the shelf life of blood bags, and the safe use of the irradiated red cells therein.

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Title: The effect of gamma radiation on the lipid profile of irradiated red blood cells
Authors: Grazielle Aparecida Silva Maia
Cristiane de Oliveira Renó
Jorge Mansur Medina
Alan Barbosa da Silveira
Julio Alberto Mignaco
Georgia Correa Atella
Vanessa Faria Cortes
Leandro Augusto Barbosa
Hérica de Lima Santos
Publication date: 01-05-2014
Publisher: Springer Berlin Heidelberg
Published in: Annals of Hematology / Issue 5/2014
Print ISSN: 0939-5555
Electronic ISSN: 1432-0584
DOI: https://doi.org/10.1007/s00277-013-1944-5

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