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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2013

Open Access 01-12-2013 | Research article

The effect of Chinese Jinzhida recipe on the hippocampus in a rat model of diabetes-associated cognitive decline

Authors: Xiao-Hui Chang, Li-Na Liang, Li-Bin Zhan, Xiao-Guang Lu, Xiang Shi, Xin Qi, Zhao-Lan Feng, Mei-Juan Wu, Hua Sui, Lu-Ping Zheng, Fu-Liang Zhang, Jie Sun, Chang-Chuan Bai, Nan Li, Guo-Zhu Han

Published in: BMC Complementary Medicine and Therapies | Issue 1/2013

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Abstract

Background

To investigate the effects of treatment with Multi component Chinese Medicine Jinzhida (JZD) on behavioral deficits in diabetes-associated cognitive decline (DACD) rats and verify our hypothesis that JZD treatment improves cognitive function by suppressing the endoplasmic reticulum stress (ERS) and improving insulin signaling transduction in the rats’ hippocampus.

Methods

A rat model of type 2 diabetes mellitus (T2DM) was established using high fat diet and streptozotocin (30 mg/kg, ip). Insulin sensitivity was evaluated by the oral glucose tolerance test and the insulin tolerance test. After 7 weeks, the T2DM rats were treated with JZD. The step-down test and Morris water maze were used to evaluate behavior in T2DM rats after 5 weeks of treatment with JZD. Levels of phosphorylated proteins involved in the ERS and in insulin signaling transduction pathways were assessed by Western blot for T2DM rats’ hippocampus.

Results

Compared to healthy control rats, T2DM rats initially showed insulin resistance and had declines in acquisition and retrieval processes in the step-down test and in spatial memory in the Morris water maze after 12 weeks. Performance on both the step-down test and Morris water maze tasks improved after JZD treatment. In T2DM rats, the ERS was activated, and then inhibited the insulin signal transduction pathways through the Jun NH2-terminal kinases (JNK) mediated. JZD treatment suppressed the ERS, increased insulin signal transduction, and improved insulin resistance in the rats’ hippocampus.

Conclusions

Treatment with JZD improved cognitive function in the T2DM rat model. The possible mechanism for DACD was related with ERS inducing the insulin signal transduction dysfunction in T2DM rats’ hippocampus. The JZD could reduce ERS and improve insulin signal transduction and insulin resistance in T2DM rats’ hippocampus and as a result improved the cognitive function.
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Metadata
Title
The effect of Chinese Jinzhida recipe on the hippocampus in a rat model of diabetes-associated cognitive decline
Authors
Xiao-Hui Chang
Li-Na Liang
Li-Bin Zhan
Xiao-Guang Lu
Xiang Shi
Xin Qi
Zhao-Lan Feng
Mei-Juan Wu
Hua Sui
Lu-Ping Zheng
Fu-Liang Zhang
Jie Sun
Chang-Chuan Bai
Nan Li
Guo-Zhu Han
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2013
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-13-161

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