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Published in: Diseases of the Colon & Rectum 9/2008

01-09-2008 | Original Contribution

The Effect of a Hyaluronan-Carboxymethylcellulose Membrane vs. Polyglactin 910 Mesh on Intra-Abdominal Tumor Formation in Mice

Authors: Peter K. Lee, M.D., Andrew P. Windsperger, B.A., B.S., Christopher M. Wilson, B.A., James B. McCarthy, Ph.D., Karen R. Wasiluk, Ph.D., David A. Rothenberger, M.D., Kelli M. Bullard Dunn, M.D.

Published in: Diseases of the Colon & Rectum | Issue 9/2008

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Abstract

Purpose

Hyaluronan mediates growth of SW620 colon cancer cells. Because hyaluronan is the active ingredient in Seprafilm®, we hypothesized that Seprafilm® would affect intraperitoneal tumor growth in a mouse model of peritoneal seeding.

Methods

Immunodeficient mice underwent laparotomy and intraperitoneal inoculation of 105 SW620 cells. Seprafilm® (n = 22), Vicryl mesh (foreign body control; n = 24), or no material (sham; n = 19) was placed under the incision. Mice were killed after four weeks and tumors were dissected, counted, and weighed.

Results

Ninety-five percent of mice in the sham group and 96 percent in the Vicryl group developed intraperitoneal tumors. In contrast, only 64 percent of mice in the Seprafilm® group developed tumors (P = 0.024), and these tumors were smaller than those in the sham group; (Seprafilm® = 42 ± 9 mg vs. sham = 82 ± 17 mg; P = 0.05). In contrast, tumors in the Vicryl group were dramatically larger (349 ± 49 mg; P < 0.001 vs. sham or Seprafilm®).

Conclusions

Despite previous data that suggested that hyaluronan increases colon cancer cell growth, we found that Seprafilm® decreased tumor formation and tended to decrease size in this model. In contrast, Vicryl mesh increased tumor formation and size. Our results suggest that Seprafilm® does not promote intraperitoneal tumor growth, especially compared with Vicryl mesh.
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Metadata
Title
The Effect of a Hyaluronan-Carboxymethylcellulose Membrane vs. Polyglactin 910 Mesh on Intra-Abdominal Tumor Formation in Mice
Authors
Peter K. Lee, M.D.
Andrew P. Windsperger, B.A., B.S.
Christopher M. Wilson, B.A.
James B. McCarthy, Ph.D.
Karen R. Wasiluk, Ph.D.
David A. Rothenberger, M.D.
Kelli M. Bullard Dunn, M.D.
Publication date
01-09-2008
Publisher
Springer-Verlag
Published in
Diseases of the Colon & Rectum / Issue 9/2008
Print ISSN: 0012-3706
Electronic ISSN: 1530-0358
DOI
https://doi.org/10.1007/s10350-008-9299-z

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