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Open Access 01-12-2015 | Original research

The dog prostate cancer (DPC-1) model: a reliable tool for molecular imaging of prostate tumors and metastases

Authors: Simone Chevalier, Serge Moffett, Eric Turcotte, Murillo Luz, Lyne Chauvette, Vilma Derbekyan, Eleonora Scarlata, Fatima Zouanat, Armen G. Aprikian, Maurice Anidjar

Published in: EJNMMI Research | Issue 1/2015

Abstract

Background

Clinical applicability of newly discovered reagents for molecular imaging is hampered by the lack of translational models. As the dog prostate cancer (DPC-1) model recapitulates in dogs the natural history of prostate cancer in man, we tested the feasibility of single-photon emission computed tomography (SPECT)/CT imaging in this model using an anti-prostate-specific membrane antigen (PSMA)/17G1 antibody as the radiotracer.

Methods

Immunoblots and immunohistochemistry (IHC) with 17G1 were performed on canine and human prostate cancer cell lines and tissues. Five dogs with DPC-1 tumors were enrolled for pelvic and, in some instances, thoracic SPECT/CT procedures, also repeated over time. Controls included ¹¹¹indium (In)-17G1 prior to DPC-1 implantation and ¹¹¹In-immunoglobulins (IgGs) prior to imaging with ¹¹¹In-17G1 in dogs bearing prostatic DPC-1 tumors.

Results

17G1 cross-reactivity with canine PSMA (and J591) was confirmed by protein analyses on DPC-1, LNCaP, and PC-3 cell lines and IHC of dog vs. human prostate tissue sections. 17G1 stained luminal cells and DPC-1 cancer cells in dog prostates similarly to human luminal and cancer cells of patients and LNCaP xenografts. SPECT/CT imaging revealed low uptake (≤2.1) of both ¹¹¹In-17G1 in normal dog prostates and ¹¹¹In-IgGs in growing DPC-1 prostate tumors comparatively to ¹¹¹In-17G1 uptake of 3.6 increasing up to 6.5 values in prostate with DPC-1 lesions. Images showed a diffused pattern and, occasionally, a peripheral doughnut-shape-like pattern. Numerous sacro-iliac lymph nodes and lung lesions were detected with contrast ratios of 5.2 and 3.0, respectively. The highest values were observed in pelvic bones (11 and 19) of two dogs, next confirmed as PSMA-positive metastases.

Conclusions

This proof-of-concept PSMA-based SPECT/CT molecular imaging detecting primary prostate tumors and metastases in canines with high cancer burden speaks in favor of this large model’s utility to facilitate technology transfer to the clinic and accelerate applications of new tools and modalities for tumor staging in patients.

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Title: The dog prostate cancer (DPC-1) model: a reliable tool for molecular imaging of prostate tumors and metastases
Authors: Simone Chevalier
Serge Moffett
Eric Turcotte
Murillo Luz
Lyne Chauvette
Vilma Derbekyan
Eleonora Scarlata
Fatima Zouanat
Armen G. Aprikian
Maurice Anidjar
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: EJNMMI Research / Issue 1/2015
Electronic ISSN: 2191-219X
DOI: https://doi.org/10.1186/s13550-015-0155-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The dog prostate cancer (DPC-1) model: a reliable tool for molecular imaging of prostate tumors and metastases

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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