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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-04-2010 | Basic Science

The correlation between the regulation of recombinant human IGF-2 on eye growth and form-deprivation in guinea pig

Authors: Zhi-hong Deng, Jia Tan, Shuang-zhen Liu, Shao-zhen Zhao, Jian-tao Wang

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 4/2010

Abstract

Objective

By investigating the effects of recombinant human IGF-2 (rhIGF-2) on the diopter, axial eye length and the expression of IGF-2 in non-form-deprivation (FD) and FD eyes of guinea pig, we tried to elucidate the relationship between the effects of rhIGF-2 on eye growth and FD in guinea pig.

Methods

Eighty 3-week-old guinea pigs were included in the study, which were divided into two groups randomly. Group A (n = 40) was the non-FD group, Thirty-two guinea pigs received intravitreal injections of either 1 ng, 10 ng, 100 ng rhIGF-2 or bovine serum albumin (BSA) to the right eye; eight guinea pigs who received no intravitreal injections served as control. Group B (n = 40) was the FD group; the right eyes were form-deprived, and received the same disposals as group A. The diopter and the axial eye length of all guinea pigs were measured at day 14. The expression of IGF-2 in the retina was measured by Western blot.

Results

After 14 days, FD eyes were high myopia. The axial length of FD eyes was significant for longer than that of non-FD eyes. The expression of IGF-2 in the retina of FD eyes was up-regulated. In non-FD group, there was no significant difference in the diopter and axial eye length among rhIGF-2 injection eyes, BSA injection eyes and control eyes, but the expression of IGF-2 in the retina of 10 ng and 100 ng rhIGF-2 injection eyes was obviously down-regulated. In the FDM (form-deprivation myopia) group, the myopic diopter and axial eye length increased in 10 ng and 100 ng rhIGF-2 injection eyes compared with those in BSA injection eyes and control eyes. The level of IGF-2 expression in the retina of 10 ng and 100 ng rhIGF-2 injection eyes was obviously down-regulated. The diopter and expression of IGF-2 in the retina of rhIGF-2 injection eyes was negatively correlated with the dose of each injection, which was positively correlated with the axial length.

Conclusion

RhIGF-2 intravitreous injection does not affect the diopter and axial length in non-FD eyes of guinea pig, while it can induce a significant increase in myopic diopter and axial length of FD eyes. RhIGF-2 can promote the development of FDM on the condition of FD.

Schmid C (1995) insulin-like growth factors. Cell Biology International 19:445–457CrossRefPubMed

Baxter RC (1986) The somatomedins: insulin-like growth factors. Adv Clin Chem 25:49–115CrossRefPubMed

Brown AL, Graham DE, Nissley SP, Hill DJ, Strain AJ, Rechler MM (1986) Developmental regulation of insulin like growth factor II mRNA in different rat tissues. J Biol Chem 261:13144–13150PubMed

Lund PK, Moats-Staats BM, Hynes MA et al (1986) Somatomedin-C/insulin-like growth factor-I and insulin-like growth factor-II mRNAs in rat fetal and adult tissues. J Biol Chem 261:14539–14544PubMed

Cuthbertson RA, Beck F, Senior PV, Haralambidis J, Penschow JD, Coghlan JP (1989) Insulin-like growth factor II may play a local role in the regulation of ocular size. Development 107:123–130PubMed

Beck F, Samani NJ, Penschow JD, Thorley B, Tregear GW, Coghlan JP (1987) Histochemical localization of IGF-I and -II mRNA in the developing rat embryo. Development 101:175–184PubMed

Lofqvist C, Willett KL, Aspegren O, Smith AC, Aderman CM, Connor KM, Chen J, Hellstrom A, Smith LE (2009) Quantification and localization of the IGF/insulin system expression in retinal blood vessels and neurons during oxygen-induced retinopathy in mice. Invest Ophthalmol Vis Sci 50:1831–1837CrossRefPubMed

Kusakari T, Sato T, Tokoro T (2001) Visual deprivation stimulates the exchange of the fibrous sclera into the cartilaginous sclera in chicks. Exp Eye Res 73(4):533–546CrossRefPubMed

Tokoro T (1994) Mechanism of axial elongation and chorioretinal atrophy in high myopia. Nippon Ganka Gakkai Zasshi 98:1213–1237PubMed

10.

Deng ZH, Liu SZ, Tan J (2006) Dynamic change of IGF-1/IGF-2mRNA expression in the posterior sclera of FDM in chicken (in Chinese). Journal of Optometry & Ophthalmology 8(49–51):54

11.

Wallman J, Winawer J (2004) Homeostasis of eye growth and the question of myopia. Neuron 43:447–468CrossRefPubMed

12.

Jobling AI, Wan R, Gentle A, Bui BV, McBrien NA (2009) Retinal and choroidal TGF-beta in the tree shrew model of myopia: isoform expression, activation and effects on function. Exp Eye Res 88:458–466CrossRefPubMed

13.

Jobling AI, Nguyen M, Gentle A, McBrien NA (2004) Isoform-specific changes in scleral transforming growth factor-beta expression and the regulation of collagen synthesis during myopia progression. J Biol Chem 279:18121–18126CrossRefPubMed

14.

Rohrer B, Stell WK (1994) Basic fibroblast growth factor (bFGF) and transforming growth factor beta (TGF-beta) act as stop and go signals to modulate postnatal ocular growth in the chick. Exp Eye Res 58:553–561CrossRefPubMed

15.

Rohrer B, Tao J, Stell WK (1997) Basic fibroblast growth factor, its high- and low-affinity receptors, and their relationship to form-deprivation myopia in the chick. Neuroscience 79:775–787CrossRefPubMed

16.

Seko Y, Shimokawa H, Tokoro T (1995) Expression of bFGF and TGF-beta 2 in experimental myopia in chicks. Invest Ophthalmol Vis Sci 36:1183–1187PubMed

17.

Akamatsu S, Fujii S, Escaño MFT, Ishibashi K, Sekiya Y, Yamamoto M (2001) Altered expression of genes in experimentally induced myopic chick eyes. Jpn J Ophthalmol 45:137–143CrossRefPubMed

18.

Seko Y, Tanaka Y, Tokoro T (1995) Influence of bFGF as a potent growth stimulator and TGF-beta as a growth regulator on scleral chondrocytes and scleral fibroblasts in vitro. Ophthalmic Res 27:144–152CrossRefPubMed

19.

Liu GL, Jiang SW, Xiong YZ et al (2002) The character of heredity and biological function of insulin-like growth factor 2. Yi Chuan 24:211–213PubMed

20.

Maachi F, Straczek J, Jacob C et al (1995) A seric low molecular weight factor modulates IGFs effects on chick cartilage metabolism. Life Sci 56:2273–2284CrossRefPubMed

21.

Deng ZH, Liu SZ, Tan J (2005) IGF-1R/IGF-2RmRNA expression in the posterior sclera of chicken form-deprivation myopia (in chinese). International Journal of Ophthalmology 5(929):931

Title: The correlation between the regulation of recombinant human IGF-2 on eye growth and form-deprivation in guinea pig
Authors: Zhi-hong Deng
Jia Tan
Shuang-zhen Liu
Shao-zhen Zhao
Jian-tao Wang
Publication date: 01-04-2010
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 4/2010
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-009-1287-z

At a glance: The STEP trials

Springer Medicine

The correlation between the regulation of recombinant human IGF-2 on eye growth and form-deprivation in guinea pig

Abstract

Objective

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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