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European Journal of Nutrition
Published in: European Journal of Nutrition 4/2011

01-06-2011 | Original Contribution

The contribution of α1B-adrenoceptor subtype in the renal vasculature of fructose-fed Sprague–Dawley rats

Authors: Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Md. Abdul Hye Khan, Kolla R. L. Anand Swarup, Edward J. Johns

Published in: European Journal of Nutrition | Issue 4/2011

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Abstract

Purpose

Fructose feeding induces a moderate increase in blood pressure, insulin resistance, and hyperinsulinemia. This study investigated the role of α1B-adrenoceptor subtype in the control of renal hemodynamic responses to exogenously administered angiotensin II (Ang II) and a set of adrenergic agonists in a model of high fructose-fed rats.

Methods

Sprague–Dawley rats were fed for 8 weeks with 20% fructose in drinking water (FFR). The renal cortical vasoconstriction to noradrenaline (NA), phenylephrine (PE), methoxamine (ME) and Ang II in the presence and absence of chloroethylclonidine (CEC) (α1B-adrenoceptor antagonist) was determined. Data, mean ± SEM or SD were subjected to ANOVA with significance at p < .05.

Results

FFR showed significant increase in the systolic blood pressure, plasma glucose, and insulin levels when compared to control. FFR expressed reduced renal cortical vascular sensitivity to NA, PE, ME, and Ang II. Furthermore, renal cortical vasoconstriction response to NA, PE, ME, and Ang II was blunted in the presence of CEC in control. While in FFR, renal cortical vasoconstriction to NA, PE, and ME was enhanced by CEC. Renal cortical vasoconstriction to Ang II in FFR was reduced in the presence of CEC.

Conclusions

In the presence of a hyperinsulinemic state resulting from chronic and high fructose feeding, an attenuated AT1 and α1-adrenoceptors response to Ang II and adrenergic stimuli respectively, is expected. In addition, α1B-adrenoceptor is the functional subtype that mediates renal cortical vasoconstriction in control rat, while high fructose feeding did influence the functionality of α1B-adrenoceptor in mediating the renal cortical hemodynamic changes.
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Metadata
Title
The contribution of α1B-adrenoceptor subtype in the renal vasculature of fructose-fed Sprague–Dawley rats
Authors
Mohammed H. Abdulla
Munavvar A. Sattar
Nor A. Abdullah
Md. Abdul Hye Khan
Kolla R. L. Anand Swarup
Edward J. Johns
Publication date
01-06-2011
Publisher
Springer-Verlag
Published in
European Journal of Nutrition / Issue 4/2011
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-010-0133-8

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