Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Virology Journal
Published in: Virology Journal 1/2009

Open Access 01-12-2009 | Research

The complete genomic sequence of an in vivo low replicating BLV strain

Authors: Syamalima Dube, Lynn Abbott, Dipak K Dube, Guillermina Dolcini, Silvina Gutierrez, Carolina Ceriani, Marcela Juliarena, Jorge Ferrer, Raisa Perzova, Bernard J Poiesz

Published in: Virology Journal | Issue 1/2009

Login to get access

Abstract

DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
Appendix
Available only for authorised users
Literature
1.
Ferrer JF: Bovine lymphosarcoma. Adv Vet Sci Comp Med 1980, 24: 1-68.PubMed
2.
Brenner J, Van Hamm M, Savir D, Trainin Z: The implication of BLV infection in the productivity, reproductive capacity and survival rate of a dairy cow. Vet Immunol Immunopathol 1989, 22: 299-305. 10.1016/0165-2427(89)90017-2CrossRefPubMed
3.
Burny A, Cleuter Y, Kettman R, Mammericx M, Marbaix G, Portelle D, Broeke A, Willems L, Thomas R: Bovine Leukemia: Facts and hypotheses derived from the study of an infectious cancer. In "Retrovirus Biology and Human Disease". Edited by: Gallo R, Wong-Stahl F. Dekker, New York; 1990:9-32.
4.
Trono K, Pérez-Filgueira D, Duffy S, Costelli M, Lager I, Duffy S, Borca MV, Carrillo C: "Virus de Leucosis Bovina: Epidemiologica en Argentina." VI Congresso Argentino de Virologica. 1999.
5.
Poiesz B, Poiesz M, Choi D: Human T-cell lymphoma/leukemia virus-associated T-cell lymphoma and leukemia. In "Neoplastic Diseases of the Blood". Edited by: Wiernik P, Goldman J, Dutcher J, Kyles R. Cambridge University Press, Cambridge, UK; 2003:141-162.
6.
Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F, Balon H, Bouzar AB, Defoiche J, Burny A, Reichert M, Kettmann R, Willems L: Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in humans. Retrovirology 2007, 4: 18-49. 10.1186/1742-4690-4-18PubMedCentralCrossRefPubMed
7.
Saksena NK, Herve V, Durand JP, Leguenno B, Diop OM, Digouette JP, Mathiot C, Muller MC, Love JL, Dube S, Sherman M, Benz P, Erensoy S, Galat-Wong A, Galat G, Paul B, Dube D, Barre'-Sinoussi F, Poiesz B: Seroepidemiologic, molecular, and phylogenetic analyses of simian T-cell leukemia viruses (STLV-I) from various naturally infected monkey species from central and western Africa. Virology 1994, 198: 297-310. 10.1006/viro.1994.1033CrossRefPubMed
8.
Poiesz BJ, Dube S, Choi D, Esteban E, Ferrer J, Leon-Ponte M, de Perez GE, Glaser J, Devare SG, Vallari AS, Schochetman G: Comparative performances of an HTLV-I/II EIA and other serologic and PCR assays on samples from persons at risk for HTLV-II infection. Transfusion 2000, 40: 924-930. 10.1046/j.1537-2995.2000.40080924.xCrossRefPubMed
9.
Gutierrez S, Dolcini G, Arroyo G, Rodriquez D, Ferrer J, Esteban E: Development and evaluation of a highly sensitive and specific blocking enzyme-linked immunosorbent assay and polymerase chain reaction assay for diagnosis of bovine leukemia virus infection in cattle. Am J Vet Res 2001, 62: 1571-1577. 10.2460/ajvr.2001.62.1571CrossRefPubMed
10.
Juliarena MA, Gutierrez SE, Ceriani C: Determination of proviral load in bovine leukemia virus-infected cattle with and without lymphocytosis. Am J Vet Res 2007, 68: 1220-5. 10.2460/ajvr.68.11.1220CrossRefPubMed
11.
Dube S, Dolcini G, Abbott L, Mehta S, Dube D, Gutierrez S, Ceriani C, Esteban E, Ferrer J, Poiesz B: The complete genomic sequence of a BLV strain from a Holstein cow from Argentina. Virology 2000, 277: 379-386. 10.1006/viro.2000.0622CrossRefPubMed
12.
Calomme C, Dekoninck A, Nizet S, Adam E, Nguyen T, Van den B, Willems L, Kettman R, Burny A, Van Lint C: Overlapping CRE and E box motifs in the enhancer sequences of the bovine leukemia virus 5' long terminal repeat are critical for basal and acetylation – dependent transcriptional activity of the viral promoter: implications for viral latency. J Virol 2004, 78: 13848-13864. 10.1128/JVI.78.24.13848-13864.2004PubMedCentralCrossRefPubMed
13.
Willems L, Kerkhofs P, Attenelle L, Burny A, Portetelle D, Kettmann R: The major homology region of bovine leukaemia virus p24 gag is required for virus infectivity in vivo. J Gen Virol 1997, 78: 637-640.CrossRefPubMed
14.
Mager A, Masengo R, Mammerickx M, Letesson JJ: T cell proliferative response to bovine leukaemia virus (BLV): identification of T cell epitopes on the major core protein (p24) in BLV-infected cattle with normal haematological values. J Gen Virol 1994, 78: 637-640.
15.
Rice N, Stephens R, Gilden R: Sequence analysis of the bovine leukemia virus genome. In "Enzootic Bovine Leukosis and Bovine Leukemia Virus". Edited by: Mammerichx A. Nijhoff, Boston; 1987:115-144.CrossRef
16.
Perzova RN, Loughran TP, Dube S, Ferrer J, Esteban E, Poiesz BJ: Lack of BLV and PTLV DNA sequences in the majority of patients with large granular lymphocyte leukaemia. Br J Haematol 2000, 109: 64-70. 10.1046/j.1365-2141.2000.01972.xCrossRefPubMed
17.
Kettman R, Burny A, Callebaut I, Droogmans L, Mammericx M, Wilems L, Portetelle D: Bovine leukemia virus. In "Retroviridae,". Volume 3. Edited by: Levy J. Plenum, New York; 1994:39-81.CrossRef
18.
Sakurai M, Taneda A, Nagoya H, Sekikawa K: Construction and functional characterization of mutants of the bovine leukemia virus trans-activator protein p34 tax. J Gen Virol 1991, 72: 2527-2531. 10.1099/0022-1317-72-10-2527CrossRefPubMed
19.
Alexandersen S, Carpenter S, Christensen J, Storgaard T, Viuff B, Wannemuhler Y, Belousov J, Roth J: Identification of alternatively spliced m-RNAs encoding potential new regulatory proteins in cattle infected with bovine leukemia virus. J Virol 1993, 67: 39-52.PubMedCentralPubMed
20.
Juliarena MA, Poli M, Sala L, Ceriani C, Gutierrez S, Dolcini G, Rodríguez EM, Mariño B, Rodríguez-Dubra C, Esteban EN: Association of BLV infection profiles with alleles of the BoLA-DRB3.2 gene. Anim Genet 2008, 39: 432-438. 10.1111/j.1365-2052.2008.01750.xCrossRefPubMed
21.
Esteban EN, Poli M, Poiesz B, Ceriani C, Dube S, Guterrez S, Dolcini G, Perez S, Lützelschwab C, Juliarena MA: Bovine leukemia virus (BLV) proposed control and eradication programs by marker assisted breeding of genetically resistant cattle. In "Animal Genetics". Edited by: Rechi L. Nova, Hauppage, NY; 2009:in press.
22.
Dube S, Bachman S, Spicer T, Love J, Esteban E, Ferrer J, Poiesz B: Degenerate and specific PCR assays for BLV and PTLV pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world. J Gen Virol 1997, 150: 147-153.
23.
Needeman SB, Wunsch CD: A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol 1970, 48: 443-453. 10.1016/0022-2836(70)90057-4CrossRef
24.
25.
Sagata N, Yasunaga T, Tsuzuku-Kawamura J, Ohishi K, Ogawa Y, Ikawa Y: Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses. Proc Nal Acad Sci USA 1985, 82: 677-681. 10.1073/pnas.82.3.677CrossRef
26.
Coulston J, Naif H, Brandon R, Kumar S, Khan S, Danile RC, Laven MD: Molecular cloning and sequencing of an Australian isolate of proviral bovine leukemia virus DNA comparison with other isolates. J Gen Virol 1990, 71: 1737-1748. 10.1099/0022-1317-71-8-1737CrossRefPubMed
Metadata
Title
The complete genomic sequence of an in vivo low replicating BLV strain
Authors
Syamalima Dube
Lynn Abbott
Dipak K Dube
Guillermina Dolcini
Silvina Gutierrez
Carolina Ceriani
Marcela Juliarena
Jorge Ferrer
Raisa Perzova
Bernard J Poiesz
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2009
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-6-120

Other articles of this Issue 1/2009

Virology Journal 1/2009 Go to the issue

Research

A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum

Research

Screening of specific diagnostic peptides of swine hepatitis E virus

Review

Replication of porcine circoviruses

Methodology

A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Research

Prophylactic and therapeutic activity of fully human monoclonal antibodies directed against Influenza A M2 protein

Research

Hepatitis C Virus infection in apparentenly healthy individuals with family history of diabetes in Vom, Plateau State Nigeria
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Image Credits