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01-07-2013 | Maternal-Fetal Medicine

The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth

Authors: Changchang Ye, Sayaka Katagiri, Naoyuki Miyasaka, Pariksha Bharti, Hiroaki Kobayashi, Yasuo Takeuchi, Yoshihito Momohara, Masaki Sekiguchi, Satoko Takamine, Toshiyuki Nagasawa, Yuichi Izumi

Published in: Archives of Gynecology and Obstetrics | Issue 1/2013

Abstract

Purpose

Periodontal disease is considered to be a risk factor for threatened preterm labor (TPL) and preterm birth (PB), but pathogenic mechanisms have not yet been elucidated. We hypothesized that infection with periodontopathic bacteria may enhance thrombosis through molecular mimicry with TLRVYK peptides on beta-2 glycoprotein I, a target molecule in anti-phospholipid syndrome. This study aimed to examine the effects of periodontitis on TPL and PB.

Methods

Ninety-five pregnant women (47 TPL and 48 healthy subjects) participated. Periodontal clinical parameters and periodontopathic bacteria were examined. Molecular mimicry between TLRVYK peptides and homologous peptides on the periodontopathic bacteria was examined by enzyme-linked immunosorbent assay (ELISA) using rabbit polyclonal antibodies specific for the respective peptides (SIRVYK on Aggregatibacter actinomycetemcomitans, TLRIYT on Porphyromonus gingivalis, and TLALYK on Treponema denticola). Serum high-sensitivity C-reactive protein, anti-TLRVYK and anti-SIRVYK IgG antibodies were measured using ELISA.

Results

Among the rabbit antibodies specific for the bacterial homologous peptides, only anti-SIRVYK IgG antibody reacted with TLRVYK peptides. Multivariable analysis showed that anti-SIRVYK IgG antibody was significantly associated with diagnosis of TPL. Of 95 births, 14 (14.7 %) delivered preterm. The preterm birth rate was higher in the anti-SIRVYK IgG antibody >median group than in the ≤median group. Of the 47 TPL subjects 13 had PB, and ordinal logistic regression analysis revealed that past smoking, presence of P. gingivalis and anti-SIRVYK IgG antibody were significantly correlated with PB.

Conclusions

Infection with P. gingivalis and the antibody response to SIRVYK might be associated with TPL and PB.

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Title: The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth
Authors: Changchang Ye
Sayaka Katagiri
Naoyuki Miyasaka
Pariksha Bharti
Hiroaki Kobayashi
Yasuo Takeuchi
Yoshihito Momohara
Masaki Sekiguchi
Satoko Takamine
Toshiyuki Nagasawa
Yuichi Izumi
Publication date: 01-07-2013
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 1/2013
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-013-2741-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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