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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2012

Open Access 01-12-2012 | Research

TGF beta1 and related-Smads contribute to pulmonary metastasis of hepatocellular carcinoma in mice model

Authors: Guo-Cai Li, Qing-Hai Ye, Qiong-Zhu Dong, Ning Ren, Hu-Liang Jia, Lun-Xiu Qin

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2012

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Abstract

Background

Recent studies indicate that Transforming Growth Factor beta (TGF β) correlated with pulmonary metastasis of cancers. However, the correlation between TGF β and pulmonary metastasis of hepatocellular carcinoma (HCC) is till unknown.

Methods

We detected the in vitro and in vivo expression levels of TGF β1/Smads by Real-time PCR and Western blot in MHCC97-H and MHCC97–L cell lines, which are HCC cell lines and have higher and lower pulmonary metastatic potential respectively.

Results

TGF β1 mRNA level in MHCC97-L tumors were higher than that in MHCC97-H tumors, (2.81±1.61 vs. 1.24±0.96, P=0.002), TGF β1 protein level in MHCC97-L tumors were also higher than that in MHCC97-H tumors (1.37±0.95 vs. 0.32±0.22, P<0.001). In addition, the TGF β1 mRNA level positively correlated with pulmonary metastasis, and the relations between TGF β1 and Smads were also found (R2=0.12 and 0.40, respectively).

Conclusions

Our results suggest that TGF β/ Smads promote pulmonary metastasis of HCC.
Appendix
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Metadata
Title
TGF beta1 and related-Smads contribute to pulmonary metastasis of hepatocellular carcinoma in mice model
Authors
Guo-Cai Li
Qing-Hai Ye
Qiong-Zhu Dong
Ning Ren
Hu-Liang Jia
Lun-Xiu Qin
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2012
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/1756-9966-31-93

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