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International Urogynecology Journal

Published in:

01-03-2012 | Original Article

TGF-beta 1 is a potential regulator of vaginal tropoelastin production

Authors: Peter Takacs, Yanping Zhang, Sujata Yavagal, Keith Candiotti, Nahida Chakhtoura, Carlos A. Medina

Published in: International Urogynecology Journal | Issue 3/2012

Abstract

Introduction and hypothesis

Our aims were to correlate transforming growth factor (TGF)-β1 and elastin mRNA expression in the vagina of women and to measure the effects of TGF-β1 on vaginal smooth muscle cell (SMC) proliferation and tropoelastin production.

Methods

Vaginal walls were sampled in women (n = 20). TGF-β1 and elastin mRNA expression was assessed by RT-PCR. SMC cultures were performed from vaginal wall biopsies. SMC were incubated with TGF-β1, and cell proliferation was assessed by MTT-assay. Tropoelastin production was measured by the Fastin Elastin Assay.

Results

There was a significant positive correlation between TGF-β1 and elastin mRNA (r = 0.784, P < 0.01). SMC proliferation was significantly increased by 10 ng/mL TGF-β1 [relative cell number, mean ± SD, 198% ± 32% of control (P = 0.01)]. Tropoelastin production was significantly increased by TGF-β1 [mean ± SD, 645% ± 180% of control (P = 0.01)].

Conclusions

There is a positive correlation between TGF-β1 and elastin mRNA expression in the vaginal wall. In vitro, TGF-β1 increases vaginal tropoelastin production in vaginal SMC.

Drutz HP, Alarab M (2006) Pelvic organ prolapse: demographics and future growth prospects. Int Urogynecol J Pelvic Floor Dysfunct 17(Suppl 1):S6–S9PubMed

Mant J, Painter R, Vessey M (1997) Epidemiology of genital prolapse: observations from the Oxford Family Planning Association Study. Br J Obstet Gynaecol 104:579–585PubMedCrossRef

Weber AM, Buchsbaum GM, Chen B, Clark AL, Damaser MS, Daneshgari F, Davis G, DeLancey J, Kenton K, Weidner AC, Word RA (2004) Basic science and translational research in female pelvic floor disorders: proceedings of an NIH-sponsored meeting. Neurourol Urodyn 23:288–301PubMedCrossRef

Nygaard I, Barber MD, Burgio KL, Kenton K, Meikle S, Schaffer J, Spino C, Whitehead WE, Wu J, Brody DJ (2008) Prevalence of symptomatic pelvic floor disorders in US women. Jama 300:1311–1316PubMedCrossRef

Olsen AL, Smith VJ, Bergstrom JO, Colling JC, Clark AL (1997) Epidemiology of surgically managed pelvic organ prolapse and urinary incontinence. Obstet Gynecol 89:501–506PubMedCrossRef

Handa VL, Cundiff G, Chang HH, Helzlsouer KJ (2008) Female sexual function and pelvic floor disorders. Obstet Gynecol 111:1045–1052PubMedCrossRef

Chen BH, Wen Y, Li H, Polan ML (2002) Collagen metabolism and turnover in women with stress urinary incontinence and pelvic prolapse. Int Urogynecol J Pelvic Floor Dysfunct 13:80–87, discussion 87PubMedCrossRef

Takacs P, Gualtieri M, Nassiri M, Candiotti K, Fornoni A, Medina CA (2010) Differential expression of smooth muscle regulatory proteins in the uterosacral ligaments of women with uterine prolapse. Am J Obstet Gynecol 202(620):e1–e5PubMed

Takacs P, Gualtieri M, Nassiri M, Candiotti K, Fornoni A, Medina CA (2009) Caldesmon expression is decreased in women with anterior vaginal wall prolapse: a pilot study. Int Urogynecol J Pelvic Floor Dysfunct 20:985–990PubMedCrossRef

10.

Takacs P, Nassiri M, Gualtieri M, Candiotti K, Medina CA (2009) Uterosacral ligament smooth muscle cell apoptosis is increased in women with uterine prolapse. Reprod Sci 16:447–452PubMedCrossRef

11.

Wen Y, Polan ML, Chen B (2006) Do extracellular matrix protein expressions change with cyclic reproductive hormones in pelvic connective tissue from women with stress urinary incontinence? Hum Reprod 21:1266–1273PubMedCrossRef

12.

Zong W, Stein SE, Starcher B, Meyn LA, Moalli PA (2010) Alteration of vaginal elastin metabolism in women with pelvic organ prolapse. Obstet Gynecol 115:953–961PubMedCrossRef

13.

Karam JA, Vazquez DV, Lin VK, Zimmern PE (2007) Elastin expression and elastic fibre width in the anterior vaginal wall of postmenopausal women with and without prolapse. BJU Int 100:346–350PubMedCrossRef

14.

Klutke J, Ji Q, Campeau J, Starcher B, Felix JC, Stanczyk FZ, Klutke C (2008) Decreased endopelvic fascia elastin content in uterine prolapse. Acta Obstet Gynecol Scand 87:111–115PubMedCrossRef

15.

Goepel C (2008) Differential elastin and tenascin immunolabeling in the uterosacral ligaments in postmenopausal women with and without pelvic organ prolapse. Acta Histochem 110:204–209PubMedCrossRef

16.

Liu JM, Davidson JM (1988) The elastogenic effect of recombinant transforming growth factor-beta on porcine aortic smooth muscle cells. Biochem Biophys Res Commun 154:895–901PubMedCrossRef

17.

Morty RE, Konigshoff M, Eickelberg O (2009) Transforming growth factor-beta signaling across ages: from distorted lung development to chronic obstructive pulmonary disease. Proc Am Thorac Soc 6:607–613PubMedCrossRef

18.

Zaher H, Shaker OG, EL-Komy MH, El-Tawdi A, Fawzi M, Kadry D (2009) Serum and tissue expression of transforming growth factor beta 1 in psoriasis. J Eur Acad Dermatol Venereol 23:406–409PubMedCrossRef

19.

Shindel AW, Lin G, Ning H, Banie L, Huang YC, Liu G, Lin CS, Lue TF (2010) Pentoxifylline attenuates transforming growth factor-beta1-stimulated collagen deposition and elastogenesis in human tunica albuginea-derived fibroblasts part 1: impact on extracellular matrix. J Sex Med 7:2077–2085PubMed

20.

Zuckerwise L, Banks E, Zhu C, Brouwer-Visser J, Huang GS (2009) Transforming growth factor-beta 1 overexpression in pelvic organ prolapse. J Pelvic Med Surg 12:266

21.

Lin G, Ning H, Wang G, Banie L, Lue TF, Lin CS (2010) Effects of birth trauma and estrogen on urethral elastic fibers and elastin expression. Urology 76(1018):e8–e13PubMed

22.

Bump RC, Mattiasson A, Bo K, Brubaker LP, DeLancey JO, Klarskov P, Shull BL, Smith AR (1996) The standardization of terminology of female pelvic organ prolapse and pelvic floor dysfunction. Am J Obstet Gynecol 175:10–17PubMedCrossRef

23.

Takacs P, Zhang Y, Jaramillo S, Bardawil T, Candiotti K, Medina CA (2010) The effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation. J Smooth Muscle Res 46:9–15PubMedCrossRef

24.

Yamamoto K, Yamamoto M, Akazawa K, Tajima S, Wakimoto H, Aoyagi M (1997) Decrease in elastin gene expression and protein synthesis in fibroblasts derived from cardinal ligaments of patients with prolapsus uteri. Cell Biol Int 21:605–611PubMedCrossRef

25.

Lin G, Shindel AW, Banie L, Ning H, Huang YC, Liu G, Lin CS, Lue TF (2010) Pentoxifylline attenuates transforming growth factor-beta1-stimulated elastogenesis in human tunica albuginea-derived fibroblasts part 2: interference in a TGF-beta1/Smad-dependent mechanism and downregulation of AAT1. J Sex Med 7:1787–1797PubMedCrossRef

26.

Zhang MC, Giro M, Quaglino D Jr, Davidson JM (1995) Transforming growth factor-beta reverses a posttranscriptional defect in elastin synthesis in a cutis laxa skin fibroblast strain. J Clin Invest 95:986–994PubMedCrossRef

27.

Davidson JM, Zoia O, Liu JM (1993) Modulation of transforming growth factor-beta 1 stimulated elastin and collagen production and proliferation in porcine vascular smooth muscle cells and skin fibroblasts by basic fibroblast growth factor, transforming growth factor-alpha, and insulin-like growth factor-I. J Cell Physiol 155:149–156PubMedCrossRef

28.

Kahari VM, Olsen DR, Rhudy RW, Carrillo P, Chen YQ, Uitto J (1992) Transforming growth factor-beta up-regulates elastin gene expression in human skin fibroblasts. Evidence for post-transcriptional modulation. Lab Invest 66:580–588PubMed

29.

Paladini D, Di Spiezio SA, Mandato VD, Guerra G, Bifulco G, Mauriello S, Nappi C (2007) Association of cutis laxa and genital prolapse: a case report. Int Urogynecol J Pelvic Floor Dysfunct 18:1367–1370PubMedCrossRef

Title: TGF-beta 1 is a potential regulator of vaginal tropoelastin production
Authors: Peter Takacs
Yanping Zhang
Sujata Yavagal
Keith Candiotti
Nahida Chakhtoura
Carlos A. Medina
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 3/2012
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-011-1589-7

At a glance: The STEP trials

Springer Medicine

TGF-beta 1 is a potential regulator of vaginal tropoelastin production

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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