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European Journal of Medical Research
Published in: European Journal of Medical Research 1/2023

Open Access 01-12-2023 | Review

TGF-β promotes the proliferation and osteogenic differentiation of dental pulp stem cells a systematic review and meta-analysis

Authors: Pengfei Gao, Chanjuan Liu, Hui Dong, Qi Li, Yunfang Chen

Published in: European Journal of Medical Research | Issue 1/2023

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Abstract

Background

Dental pulp stem cells (DPSCs) are adult stem cells with multi-directional differentiation potential derived from ectoderm. Vitro experiments have shown that adding cytokines can help DPSCs to be transformed from multipotent stem cells to osteoblasts. TGF-β has been proved to have an effect on the proliferation and mineralization of bone tissue, but its effect on the osteogenesis and proliferation of dental pulp stem cells is still uncertain. We aim to determine the effect of TGF-β on the osteogenesis and proliferation of dental pulp stem cells.

Methods

We have identified studies from the Cochrane Central Register of Controlled Trials, PubMed, Embase, and China national knowledge infrastructure (CNKI) for studies interested in TGF-β and proliferation and differentiation of dental pulp stem cells in the following indicators: A490 (an index for evaluating cell proliferation), bone sialoprotein (BSP), Col plasmid-1 (Col-1), osteocalcin (OCN), runt-related transcription factor 2 (Runx-2); and the number of mineralized nodules. Any language restrictions were rejected. Furthermore, we drew a forest plot for each outcome. We conducted a sensitivity analysis, data analysis, heterogeneity, and publication bias test. We evaluate the quality of each study under the guidance of Cochrane's tool for quality assessment.

Results

The pooled data showed that TGF-β could promote the proliferation and ossification of dental pulp stem cells. All the included results support this conclusion except for the number of mineralized nodules: TGF-β increases the A490 index (SMD 3.11, 95% CI [0.54–5.69]), promotes the production of BSP (SMD 3.11, 95% CI [0.81–6.77]), promotes the expression of Col-1 (SMD 4.71, 95% CI [1.25–8.16]) and Runx-2 (SMD 3.37, 95% CI [− 0.63 to 7.36]), increases the content of OCN (SMD 4.32, 95% CI [1.20–7.44]) in dental pulp, and has no significant effect on the number of mineralized nodules (SMD 3.87, 95% CI [− 1.76 to 9.51]) in dental pulp stem cells.

Conclusions

TGF-β promotes the proliferation and osteogenesis of dental pulp stem cells.
Appendix
Available only for authorised users
Literature
1.
Marit NH, Richard TH, Lynn MM. TGF-beta and related proteins in development, an MCDB/ISU symposium. Ames, Iowa, September 20–23, 1991. Mol Reprod Dev. 1992;32(2):89–184.
2.
Huang XF, Chai Y. TGF-ß signalling and tooth development. Chin J Dent Res. 2010;13(1):7–15.PubMed
3.
Chen W, Huang X, Peng A, et al. Kangquan recipe regulates the expression of BAMBI protein via the TGF-β/Smad signaling pathway to inhibit benign prostatic hyperplasia in rats. Evid Complement Alternat Med. 2019;2019:6281819.
4.
Nuti N, Corallo C, Chan BM, et al. Multipotent differentiation of human dental pulp stem cells: a literature review. Stem Cell Rev Rep. 2016;12(5):511–23.CrossRefPubMed
5.
6.
Mizokami A, Kawakubo-Yasukochi T, Hirata M. Osteocalcin and its endocrine functions. Biochem Pharmacol. 2017;132:1–8.CrossRefPubMed
7.
Hu X, Zhong Y, Kong Y, et al. Lineage-specific exosomes promote the odontogenic differentiation of human dental pulp stem cells (DPSCs) through TGFβ1/smads signaling pathway via transfer of microRNAs. Stem Cell Res Ther. 2019;10(1):170.CrossRefPubMedPubMedCentral
8.
Chen YJ, Yao CC, Huang CH, et al. Hexosamine-induced TGF-β signaling and osteogenic differentiation of dental pulp stem cells are dependent on N-acetylglucosaminyltransferase V. Biomed Res Int. 2015;2015:924397.CrossRefPubMedPubMedCentral
9.
Qi X, Xiao Q, Sheng R, Jiang S, et al. Endogenous GDF11 regulates odontogenic differentiation of dental pulp stem cells. J Cell Mol Med. 2020;24(19):11457–64.CrossRefPubMedPubMedCentral
10.
Gopinath VK, Soumya S, Mohammad MG. Ror β expression in activated macrophages and dental pulp stem cells. Int Endod J. 2021;54(3):388–98.CrossRefPubMed
11.
Wang Y, He T, Liu J, et al. Synergistic effects of overexpression of BMP-2 and TGF-β3 on osteogenic differentiation of bone marrow mesenchymal stem cells. Mol Med Rep. 2016;14(6):5514–20.CrossRefPubMedPubMedCentral
12.
Wu Z. Effects of TGF-β_3 and dental pulp stem cells combined with Bio-OSS bone meal on bone defect repair in animals. In: Hoga M, editor. (01), 2015.
13.
Halik MI. Study on the osteogenic differentiation ability of rabbit dental pulp stem cells induced by heparin promoting transforming growth factor-β_3 in vitro. In: Hoga M, editor. (10), 2016.
14.
Khalik MI, Aini J, Xiaotao C, et al. In vitro study of the osteogenic differentiation potential of TGF-β3 on rabbit dental pulp stem cells under constant magnetic field. J Clin Stomatol. 2019;35(12):719–23.
15.
Ababekli G. Effects of local combined application of TGF-β3 and DPSCs on immediate osseointegration of rabbit implants. In: Hoga M, editor. (11), 2018.
16.
Weiqian G, Yuming Z, Ge L. Human recombinant transforming growth factor β_1 promotes the proliferation and mineralization of dental pulp stem cells. J Peking Univ (Med Sci). 2017;49(04):680–4.
17.
Alone, Hoga M, Keiko W. Effect of exogenous transforming growth factor-β_3 combined with rabbit pulp stem cells on expression of transforming growth factor-β_3 in rabbit bone defect osteoblasts. Chin J Pract Diagn Ther. 2017;31(06): 525–9.
18.
Ai X, Guitang W, Xingrong L. Effects of insulin-like growth factor-i and transforming growth factor-β1 on proliferation and differentiation of human dental pulp stem cells. Mod Prev Med. 2014;41(12):2237–40.
19.
Liming J, Ge S, Business Itso, Chen X. Effect of transforming growth factor β1 on osteogenic differentiation of human dental pulp stem cells. Chin J Pract Stomatol. 2018;11(09): 530–3.
20.
Ernival E, Zhang X, Maimamtimin K, et al. In vitro study of transforming growth factor β3 promoting osteogenic differentiation of rabbit dental pulp stem cells. J Pract Stomatol. 2019;35(04):485–9.
21.
Fei R, Jinmei L, et al. Effects of transforming growth factor β3 on proliferation and mineralization of human deciduous pulp stem cells. Chin Tissue Eng Res. 2014;18(28):4542–8.
22.
Liu X, Hoga M, Khalik MI, et al. Effects of transforming growth factor-β_3 on proliferation and osteogenic differentiation of rabbit dental pulp stem cells. Chin J Pract Diagn Ther. 2016;30(03):242–5.
23.
Fei R, Hui SD, et al. effect of transforming growth factor β3 combined with heparin on differentiation of human deciduous dental pulp stem cells into odontoblast-like cells. J Pract Med. 2014;30(12):1887–90.
24.
25.
26.
Lodyga M, Hinz B. TGF-β1—a truly transforming growth factor in fibrosis and immunity. Semin Cell Dev Biol. 2020;101:123–39.CrossRefPubMed
27.
28.
Mu M, Zuo S, Wu RM, et al. Ferulic acid attenuates liver fibrosis and hepatic stellate cell activation via inhibition of TGF-β/Smad signaling pathway. Drug Des Dev Ther. 2018;12:4107–15.CrossRef
29.
Lizhi Z, Zhen W, Zhang S. The role of transforming growth factor β signaling in osteoarthritis. Chin J Bone Joint Surg. 2019;12(09):727–32.
30.
Huang GT, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009;88(9):792–806.CrossRefPubMedPubMedCentral
31.
Wu M, Chen G, Li YP. TGF-β and BMP signaling in osteoblast, skeletal development, and bone formation, homeostasis and disease. Bone Res. 2016;4:16009.CrossRefPubMedPubMedCentral
32.
33.
Metadata
Title
TGF-β promotes the proliferation and osteogenic differentiation of dental pulp stem cells a systematic review and meta-analysis
Authors
Pengfei Gao
Chanjuan Liu
Hui Dong
Qi Li
Yunfang Chen
Publication date
01-12-2023
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue 1/2023
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/s40001-023-01227-y

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