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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2022

01-12-2022 | Tendinopathy | Research

Antioxidant effect of nicotinamide mononucleotide in tendinopathy

Authors: Kohei Yamaura, Yutaka Mifune, Atsuyuki Inui, Hanako Nishimoto, Takashi Kurosawa, Shintaro Mukohara, Yuichi Hoshino, Takahiro Niikura, Ryosuke Kuroda

Published in: BMC Musculoskeletal Disorders | Issue 1/2022

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Abstract

Background

A link between tendinopathy and oxidative stress has been recently reported. Nicotinamide mononucleotide (NMN) is a precursor of nicotinamide adenine dinucleotide, which plays an important role in cell redox homeostasis. The aim of this study was to evaluate the antioxidant effect of NMN on tendinopathy in vitro and in vivo.

Methods

Tenocytes from healthy Sprague-Dawley rats were cultured in regular glucose (RG) and high-glucose (HG) conditions with or without NMN, and were divided into four groups: RG NMN(−), RG NMN(+), HG NMN(−), and HG NMN(+). Cell viability, reactive oxygen species (ROS) accumulation, apoptotic rate, and mRNA expression of nicotinamide adenine dinucleotide phosphate oxidase (NOX)1, NOX4, interleukin (IL)6, sirtuin (SIRT)1, and SIRT6 were investigated. In addition, rats with collagenase-induced tendinopathy were treated with or without NMN. Immunostaining of NOX1 and NOX4; mRNA expression of SIRT1, SIRT6, and IL6; and superoxide dismutase (SOD) activity measurements in the Achilles tendon were performed.

Results

NMN increased the expression of SIRT1 and SIRT6 in rat tenocytes, but decreased the levels of NOX1, NOX4, IL6, ROS, and apoptosis. In Achilles tendons with collagenase-induced tendinopathy, NMN increased the mRNA expression of SIRT1 and SIRT6, as well as SOD activity; while suppressing protein expression of NOX1 and NOX4, and mRNA expression of IL6.

Conclusion

The in vitro and in vivo results of this study show that NMN exerts an antioxidant effect on tendinopathy by promoting the expression of SIRT while inhibiting that of NOX.
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Metadata
Title
Antioxidant effect of nicotinamide mononucleotide in tendinopathy
Authors
Kohei Yamaura
Yutaka Mifune
Atsuyuki Inui
Hanako Nishimoto
Takashi Kurosawa
Shintaro Mukohara
Yuichi Hoshino
Takahiro Niikura
Ryosuke Kuroda
Publication date
01-12-2022
Publisher
BioMed Central
Keyword
Tendinopathy
Published in
BMC Musculoskeletal Disorders / Issue 1/2022
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-022-05205-z

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