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Knee Surgery, Sports Traumatology, Arthroscopy

Published in:

01-09-2004 | Experimental Study

Temporal expression of fibroblast growth factor receptors during primary ligament repair

Authors: Simon M. Cool, Charles P. Snyman, Victor Nurcombe, Mark Forwood

Published in: Knee Surgery, Sports Traumatology, Arthroscopy | Issue 5/2004

Abstract

Following injury, it is inherently difficult to completely restore the biomechanical properties of ligaments. Relatively little is known about the cellular mechanisms controlling ligament healing. Numerous studies have implicated fibroblast growth factors (FGFs) as key molecules during the initiation of the cellular proliferation, differentiation, migration and matrix deposition that characterise wound healing. While current surgical emphasis concentrates on growth factor intervention, the role of their cognate receptors (FGFRs) has largely been overlooked. Following transection of the medial collateral ligament (MCL) in rabbits, we examined FGFR expression over a 14-day healing period. Using semi-quantitative RT-PCR, we observed a significant upregulation in FGFR2 expression after 3 days. By 7 days post injury, FGFR2 expression fell to basal levels in line with those of FGFR1 and 3, both of which remained unaffected by surgical transection. These results demonstrate a role for FGFR2 in fibroblast and endothelial cell proliferation in damaged ligament, and suggest a window for FGF therapy.

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Title: Temporal expression of fibroblast growth factor receptors during primary ligament repair
Authors: Simon M. Cool
Charles P. Snyman
Victor Nurcombe
Mark Forwood
Publication date: 01-09-2004
Publisher: Springer-Verlag
Published in: Knee Surgery, Sports Traumatology, Arthroscopy / Issue 5/2004
Print ISSN: 0942-2056
Electronic ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-003-0444-x

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Temporal expression of fibroblast growth factor receptors during primary ligament repair

Abstract

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Abstract

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