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BMC Cancer
Published in: BMC Cancer 1/2019

Open Access 01-12-2019 | Tadalafile | Research article

Repurposing mechanistic insight of PDE-5 inhibitor in cancer chemoprevention through mitochondrial-oxidative stress intervention and blockade of DuCLOX signalling

Authors: Manjari Singh, Sweta Kasna, Subhadeep Roy, Sara Aldosary, Abdulaziz S. Saeedan, Mohd. Nazam Ansari, Gaurav Kaithwas

Published in: BMC Cancer | Issue 1/2019

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Abstract

Background

This study evaluates the anti-cancer effects of Tadalafil (potent PDE-5 inhibitor) in female albino wistar rats against n-methyl n-nitrosourea induced mammary gland carcinogenesis.

Methods

The animals were selected and randomly divided among four groups and each group contains six animals per group. The animal tissue and serum samples were evaluated for the presence of antioxidant parameters and the cellular morphology was studied using carminic staining, haematoxylin staining and scanning electron microscopy followed by immunoblotting analysis.

Results

On the grounds of hemodynamic recordings and morphology, n-methyl n-nitrosourea treated group showed distorted changes along with distorted morphological parameters. For morphological analysis, the mammary gland tissues were evaluated using scanning electron microscopy, whole mount carmine staining, haematoxylin and eosin staining. The serum samples were evaluated for the evaluation of oxidative stress markers and inflammatory markers. The level of caspase 3 and 8 were also evaluated for the estimation of apoptosis. The fatty acid profiling of mammary gland tissue was evaluated using fatty acid methyl esters formation. The mitochondrial mediated apoptosis and inflammatory markers were evaluated using immunoblotting assay.

Conclusion

The results confirm that Tadalafil treatment restored all the biological markers to the normal and its involvement in mitochondrial mediated death apoptosis pathway along with inhibition of inflammatory markers.
Appendix
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Metadata
Title
Repurposing mechanistic insight of PDE-5 inhibitor in cancer chemoprevention through mitochondrial-oxidative stress intervention and blockade of DuCLOX signalling
Authors
Manjari Singh
Sweta Kasna
Subhadeep Roy
Sara Aldosary
Abdulaziz S. Saeedan
Mohd. Nazam Ansari
Gaurav Kaithwas
Publication date
01-12-2019
Publisher
BioMed Central
Keyword
Tadalafile
Published in
BMC Cancer / Issue 1/2019
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-019-6152-9

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