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01-03-2006 | Contrast Media

T1 and T2 relaxivity of intracellular and extracellular USPIO at 1.5T and 3T clinical MR scanning

Authors: Gerhard H. Simon, Jan Bauer, Olaf Saborovski, Yanjun Fu, Claire Corot, Michael F. Wendland, Heike E. Daldrup-Link

Published in: European Radiology | Issue 3/2006

Abstract

In this study we evaluated the effects of intracellular compartmentalization of the ultrasmall superparamagnetic iron oxide (USPIO) ferumoxtran-10 on its proton T1 and T2 relaxivities at 1.5 and 3T. Monocytes were labeled with ferumoxtran-10 by simple incubation. Decreasing quantities of ferumoxtran-10-labeled cells (2.5×10⁷-0.3×10⁷ cells/ml) and decreasing concentrations of free ferumoxtran-10 (without cells) in Ficoll solution were evaluated with 1.5 and 3T clinical magnetic resonance (MR) scanners. Pulse sequences comprised axial spin echo (SE) sequences with multiple TRs and fixed TE and SE sequences with fixed TR and increasing TEs. Signal intensity measurements were used to calculate T1 and T2 relaxation times of all samples, assuming a monoexponential signal decay. The iron content in all samples was determined by inductively coupled plasma atomic emission spectrometry and used for calculating relaxivities. Measurements at 1.5T and 3T showed higher T1 and T2 relaxivity values of free extracellular ferumoxtran-10 as opposed to intracellularly compartmentalized ferumoxtran-10, under the evaluated conditions of homogeneously dispersed contrast agents/cells in Ficoll solution and a cell density of up to 2.5×10⁷ cells/ml. At 3T, differences in T1-relaxivities between intra- and extracellular USPIO were smaller, while differences in USPIO T2-relaxivities were similar compared with 1.5T. In conclusion, cellular compartmentalization of ferumoxtran-10 changes proton relaxivity.

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Title: T1 and T2 relaxivity of intracellular and extracellular USPIO at 1.5T and 3T clinical MR scanning
Authors: Gerhard H. Simon
Jan Bauer
Olaf Saborovski
Yanjun Fu
Claire Corot
Michael F. Wendland
Heike E. Daldrup-Link
Publication date: 01-03-2006
Publisher: Springer-Verlag
Published in: European Radiology / Issue 3/2006
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-005-0031-2

At a glance: The STEP trials

Springer Medicine

T1 and T2 relaxivity of intracellular and extracellular USPIO at 1.5T and 3T clinical MR scanning

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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