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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 3/2013

01-03-2013 | Gamete Biology

Survival and post-warming in vitro competence of human oocytes after high security closed system vitrification

Authors: N. De Munck, G. Verheyen, L. Van Landuyt, D. Stoop, H. Van de Velde

Published in: Journal of Assisted Reproduction and Genetics | Issue 3/2013

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Abstract

Purpose

To compare two vitrification methods and two warming methods for human oocyte vitrification using a high security closed device in terms of survival, fertilization and embryo development.

Methods

For vitrification, oocytes were (1) immediately placed in equilibration solution or (2) they were gradually exposed to the cryoprotectants. For warming, oocytes were placed (1) in a 25 μl preheated (37 °C) thawing solution droplet that was put at room temperature for 1 min once the oocytes were inside or (2) in a 150 μl droplet for 1 minute at 37 °C.

Results

Survival and preimplantation development were significantly lower when warming was performed in a small preheated droplet. There was no significant difference in survival and embryo development between the gradual or direct exposure to cryoprotectants.

Conclusions

Using this high security closed vitrification device a 90 % survival rate can be achieved when the oocytes are immediately warmed in a large volume at 37 °C.
Literature
1.
Almodin CG, Minguetti-Camara VC, Paixao CL, Pereira PC. Embryo development and gestation using fresh and vitrified oocytes. Hum Reprod. 2010;25(5):1192–8.PubMedCrossRef
2.
Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries. Reprod Biomed Online. 2007;14(1):72–9.PubMedCrossRef
3.
Ata B, Chian RC, Tan SL. Cryopreservation of oocytes and embryos for fertility preservation for female cancer patients. Best Pract Res Clin Obstet Gynaecol. 2010;24(1):101–12.PubMedCrossRef
4.
Bielanski A, Nadin-Davis S, Sapp T, Lutze-Wallace C. Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology. 2000;40(2):110–6.PubMedCrossRef
5.
Bielanski A, Vajta G. Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. Hum Reprod. 2009;24(10):2457–67.PubMedCrossRef
6.
Boldt J. Current results with slow freezing and vitrification of the human oocyte. Reprod Biomed Online. 2011;23(3):314–22.PubMedCrossRef
7.
Bonetti A, Cervi M, Tomei F, Marchini M, Ortolani F, Manno M. Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices. Fertil Steril. 2010;95(3):928–35.CrossRef
8.
Borini A, Bianchi V, Bonu MA, Sciajno R, Seren E, Cattoli M, et al. Evidence-based clinical outcome of oocyte slow cooling. Reprod Biomed Online. 2007;15(2):175–81.PubMedCrossRef
9.
Cao YX, Xing Q, Li L, Cong L, Zhang ZG, Wei ZL, et al. Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification. Fertil Steril. 2009;92(4):1306–11.PubMedCrossRef
10.
Cauffman G, De Rycke M, Sermon K, Liebaers I, Van de Velde H. Markers that define stemness in ESC are unable to identify the totipotent cells in human preimplantation embryos. Hum Reprod. 2009;24(1):63–70.PubMedCrossRef
11.
12.
Chen SU, Yang YS. Slow freezing or vitrification of oocytes: their effects on survival and meiotic spindles, and the time schedule for clinical practice. Taiwan J Obstet Gynecol. 2009;48(1):15–22.PubMedCrossRef
13.
Cobo A, Rubio C, Gerli S, Ruiz A, Pellicer A, Remohí J. Use of fluorescence in situ hybridization to assess the chromosomal status of embryos obtained from cryopreserved oocytes. Fertil Steril. 2001;75(2):354–60.PubMedCrossRef
14.
Cobo A, Kuwayama M, Pérez S, Ruiz A, Pellicer A, Remohí J. Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril. 2008;89(6):1657–64.PubMedCrossRef
15.
Cobo A, Vajta G, Remohí J. Vitrification of human mature oocytes in clinical practice. Reprod Biomed Online. 2009;19 Suppl 4:4385.PubMed
16.
Cobo A, Pérez S, de los Santos MJ, Meseguer M, Remohí J. Storage of human oocytes in the vapor phase of nitrogen. Fertil Steril. 2010;94(5):1903–7.PubMedCrossRef
17.
De Vos A, Van Landuyt L, Van Ranst H, Vandermonde A, D’Haese V, Sterckx J, et al. Randomized sibling-oocyte study using recombinant human hyaluronidase versus bovine-derived Sigma hyaluronidase in ICSI patients. Hum Reprod. 2008;23(8):1815–9.PubMedCrossRef
18.
Fadini R, Brambillasc F, Renzini MM, Merola M, Comi R, De Ponti E, et al. Human oocyte cryopreservation: comparison between slow and ultrarapid methods. Reprod Biomed Online. 2009;19(2):171–80.PubMedCrossRef
19.
Fuller B, Paynter S. Fundamentals of cryobiology in reproductive medicine. Reprod Biomed Online. 2004;9(6):680–91.PubMedCrossRef
20.
Gardner DK, Schoolcraft WB. In-vitro culture of human blastocysts. In: Jansen R, Mortimer D, editors. Towards reproductive certainty: fertility and genetics beyond 1999. Carnforth: Parthenon Press; 1999. p. 378–88.
21.
Gook DA, Osborn SM, Bourne H, Johnston WI. Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Hum Reprod. 1994;9(4):684–91.PubMed
22.
Grifo JA, Noyes N. Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preservation for female cancer patients. Fertil Steril. 2010;93(2):391–6.PubMedCrossRef
23.
Grout BW, Morris GJ. Contaminated liquid nitrogen vapour as a risk factor in pathogen transfer. Theriogenology. 2009;71(7):1079–82.PubMedCrossRef
24.
Herrero L, Martínez M, Garcia-Velasco JA. Current status of human oocyte and embryo cryopreservation. Curr Opin Obstet Gynecol. 2011;23(4):245–50.PubMed
25.
Homburg R, van der Veen F, Silber SJ. Oocyte vitrification–women’s emancipation set in stone. Fertil Steril. 2009;91(4 Suppl):1319–20.PubMedCrossRef
26.
Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E. High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril. 2003;80(1):223–4.PubMedCrossRef
27.
Kim TJ, Laufer LR, Hong SW. Vitrification of oocytes produces high pregnancy rates when carried out in fertile women. Fertil Steril. 2010;93(2):467–74.PubMedCrossRef
28.
Koutlaki N, Schoepper B, Maroulis G, Diedrich K, Al-Hasani S. Human oocyte cryopreservation: past, present and future. Reprod Biomed Online. 2006;13(3):427–36.PubMedCrossRef
29.
Kuwayama M, Vajta G, Ieda S, Kato O. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 2005;11(5):608–14.PubMedCrossRef
30.
Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online. 2005;11(3):300–8.PubMedCrossRef
31.
Lane M, Schoolcraft WB, Gardner DK. Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique. Fertil Steril. 1999;72(6):1073–8.PubMedCrossRef
32.
Liebermann J, Dietl J, Vanderzwalmen P, Tucker MJ. Recent developments in human oocyte, embryo and blastocyst vitrification: where are we now? Reprod Biomed Online. 2003;7(6):623–33.PubMedCrossRef
33.
Lucena E, Bernal DP, Lucena C, Rojas A, Moran A, Lucena A. Successful ongoing pregnancies after vitrification of oocytes. Fertil Steril. 2006;85(1):108–11.PubMedCrossRef
34.
Magli MC, Lappi M, Ferraretti AP, Capoti A, Ruberti A, Gianaroli L. Impact of oocyte cryopreservation on embryo development. Fertil Steril. 2010;93(2):510–6.PubMedCrossRef
35.
Martínez-Burgos M, Herrero L, Megías D, Salvanes R, Montoya MC, Cobo AC, et al. Vitrification versus slow freezing of oocytes: effects on morphologic appearance, meiotic spindle configuration, and DNA damage. Fertil Steril. 2010;95(1):374–7.PubMedCrossRef
36.
Mazur P, Seki S. Survival of mouse oocytes after being cooled in a vitrification solution to −196°C at 95° to 70,000°C/min and warmed at 610° to 118,000°C/min: A new paradigm for cryopreservation by vitrification. Cryobiology. 2011;62(1):1–7.PubMedCrossRef
37.
Moon JH, Hyun CS, Lee SW, Son WY, Yoon QH, Lim JH. Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the predication of embryonic developmental competence after ICSI. Hum Reprod. 2003;18(4):817–20.PubMedCrossRef
38.
Nottola SA, Coticchio G, Sciajno R, Gambardella A, Maione M, Scaravelli G, et al. Ultrastructural markers of quality in human mature oocytes vitrified using cryoleaf and cryoloop. Reprod Biomed Online. 2009;19 Suppl 3:17–27.PubMedCrossRef
39.
Noyes N, Knopman J, Labella P, McCaffrey C, Clark-Williams M, Grifo J. Oocyte cryopreservation outcomes including pre-cryopreservation and post-thaw meiotic spindle evaluation following slow cooling and vitrification of human oocytes. Fertil Steril. 2010;94(6):2078–82.PubMedCrossRef
40.
Noyes N, Boldt J, Nagy ZP. Oocyte cryopreservation: is it time to remove its experimental label? J Assist Reprod Genet. 2010;27(2–3):69–74.PubMedCrossRef
41.
Oakes MB, Gomes CM, Fioravanti J, Serafini P, Motta EL, Smith GD. A case of oocyte and embryo vitrification resulting in clinical pregnancy. Fertil Steril. 2008;90(5):2013–8.PubMedCrossRef
42.
Oktay K, Cil AP, Bang H. Efficiency of oocyte cryopreservation: a meta-analysis. Fertil Steril. 2006;86(1):70–80.PubMedCrossRef
43.
Paffoni A, Guarneri C, Ferrari S, Restelli L, Nicolosi AE, Scarduelli C, et al. Effects of two vitrification protocols on the developmental potential of human mature oocytes. Reprod Biomed Online. 2011;22(3):292–8.PubMedCrossRef
44.
Parmegiani L, Cognigni GE, Bernardi S, Ciampaglia W, Infante F, Pocognoli P, et al. Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration. Hum Reprod. 2008;23(8):1771–7.PubMedCrossRef
45.
Parmegiani L, Bertocci F, Garello C, Salvarani MC, Tambuscio G, Fabbri R. Efficiency of human oocyte slow freezing: results from five assisted reproduction centres. Reprod Biomed Online. 2009;18(3):352–9.PubMedCrossRef
46.
Parmegiani L, Cognigni GE, Bernardi S, Cuomo S, Ciampaglia W, Infante FE, et al. Efficiency of aseptic open vitrification and hermetical cryostorage of human oocytes. Reprod Biomed Online. 2011;23(4):505–12.PubMedCrossRef
47.
Paynter SJ. A rational approach to oocyte cryopreservation. Reprod Biomed Online. 2005;10(5):578–86.PubMedCrossRef
48.
Pickering SJ, Braude PR, Johnson MH, Cant A, Currie J. Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril. 1990;54(1):102–8.PubMed
49.
Rienzi L, Ubaldi F, Martinez F, Iacobelli M, Minasi MG, Ferrero S, et al. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum Reprod. 2003;18(6):1289–93.PubMedCrossRef
50.
Rienzi L, Romano S, Albricci L, Maggiulli R, Capalbo A, Baroni E, et al. Embryo development of fresh ‘versus’ vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study. Hum Reprod. 2010;25(1):66–73.PubMedCrossRef
51.
Rienzi L, Cobo A, Paffoni A, Scarduelli C, Capalbo A, Vajta G, Remohí J, Ragni G, Ubaldi FM. Consistent and predictable delivery rates after oocyte vitrification: an observational longitudinal cohort multicentric study. Hum Reprod. 2012;27(6):1606–12.
52.
Saragusty J, Arav A. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction. 2011;141(1):1–19.PubMedCrossRef
53.
Schoolcraft WB, Keller JL, Schlenker T. Excellent embryo quality obtained from vitrified oocytes. Reprod Biomed Online. 2009;19(6):820–3.PubMedCrossRef
54.
Seki S, Mazur P. The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology. 2009;59(1):75–82.PubMedCrossRef
55.
Smith GD, Serafini PC, Fioravanti J, Yadid I, Coslovsky M, Hassun P, et al. Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification. Fertil Steril. 2010;94(6):2088–95.PubMedCrossRef
56.
Smith GD, Motta EE, Serafini P. Theoretical and experimental basis of oocyte vitrification. Reprod Biomed Online. 2011;23(3):298–306.PubMedCrossRef
57.
Stachecki JJ, Cohen J. An overview of oocyte cryopreservation. Reprod Biomed Online. 2004;9(2):152–63.PubMedCrossRef
58.
Stoop D, Nekkebroek J, Devroey P. A survey on the intentions and attitudes towards oocyte cryopreservation for non-medical reasons among women of reproductive age. Human Reprod. 2011;26(3):655–61.CrossRef
59.
Stoop D, De Munck N, Jansen E, Platteau P, Van den Abbeel E, Verheyen G, et al. Clinical validation of a closed vitrification system in an oocyte donation programme. Reprod Biomed Online. 2012;24(2):180–5.PubMedCrossRef
60.
Tao T, Del Valle A. Human oocyte and ovarian tissue cryopreservation and its application. J Assist Reprod Genet. 2008;25(7):287–96.PubMedCrossRef
61.
62.
Van Landuyt L, De Vos A, Joris H, Verheyen G, Devroey P, Van Steirteghem A. Blastocyst formation in in vitro fertilization versus intracytoplasmic sperm injection cycles: influence of the fertilization procedure. Fertil Steril. 2005;83(5):1397–403.PubMedCrossRef
63.
Van Landuyt L, Stoop D, Verheyen G, Verpoest W, Camus M, Van de Velde H, et al. Outcome of closed blastocyst vitrification in relation to blastocyst quality: evaluation of 759 warming cycles in a single-embryo transfer policy. Hum Reprod. 2011;26(3):527–34.PubMedCrossRef
64.
Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe DL. Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy. Hum Reprod. 2001;16:2374–8.PubMed
65.
Wang WH, Meng L, Hackett RJ, Keefe DL. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination. Hum Reprod. 2001;16:1464–8.PubMedCrossRef
66.
Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe DL. The spindle observation and its relationship with fertilization after intracytoplasmic sperm injection in living human oocytes. Fertil Steril. 2001;75:348–53.PubMedCrossRef
67.
Wennerholm UB, Söderström-Anttila V, Bergh C, Aittomäki K, Hazekamp J, Nygren KG, et al. Children born after cryopreservation of embryos or oocytes: a systematic review of outcome data. Hum Reprod. 2009;24(9):2158–72.PubMedCrossRef
68.
Yoon TK, Kim TJ, Park SE, Hong SW, Ko JJ, Chung HM, et al. Live births after vitrification of oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril. 2003;79(6):1323–6.PubMedCrossRef
Metadata
Title
Survival and post-warming in vitro competence of human oocytes after high security closed system vitrification
Authors
N. De Munck
G. Verheyen
L. Van Landuyt
D. Stoop
H. Van de Velde
Publication date
01-03-2013
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 3/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-013-9930-3

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