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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2013

Open Access 01-12-2013 | Research

Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst

Authors: Xing-Wei Liang, Xiang-Shun Cui, Shao-Chen Sun, Yong-Xun Jin, Young Tae Heo, Suk Namgoong, Nam-Hyung Kim

Published in: Reproductive Biology and Endocrinology | Issue 1/2013

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Abstract

Background

Series of epigenetic events happen during preimplantation development. Therefore assistant reproduction techniques (ART) have the potential to disrupt epigenetic regulation during embryo development. The purpose of this study was to investigate whether defects in methylation patterns in blastocyst due to superovulation originate from abnormal expression of Dnmts.

Methods

Low- (6 IU) and high- (10 IU) dosage of PMSG was used to stimulate the female mice. The metaphase II(MII) oocytes, zygotes and blastocyst stage embryos were collected. Global methylation and methylation at H3K9 in zygote, and methylation at repeated sequence Line 1 and IAP in blastocysts were assayed. In addition, expression of Dnmts was examined in oocytes and zygotes.

Results

Global DNA methylation and methylation at H3K9 in zygotes derived from females after low- or high-dosage hormone treatment were unaltered compared to that in controls. Moreover, DNA methylation at IAP in blastocysts was also unaffected, regardless of hormone dosage. In contrast, methylation at Line1 decreased when high-dose hormone was administered. Unexpectedly, expression of Dnmt3a, Dnmt3b, Dnmt3L as well as maintenance Dnmt1o in oocytes and zygotes was not disrupted.

Conclusions

The results suggest that defects in embryonic methylation patterns do not originate from the disruption of Dnmt expression.
Appendix
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Metadata
Title
Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst
Authors
Xing-Wei Liang
Xiang-Shun Cui
Shao-Chen Sun
Yong-Xun Jin
Young Tae Heo
Suk Namgoong
Nam-Hyung Kim
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2013
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-11-69

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