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01-05-2018 | Original Article

Sugar-based collagen membrane cross-linking increases barrier capacity of membranes

Authors: Po-ju Chia-Lai, Anna Orlowska, Sarah Al-Maawi, Andre Dias, Yunxin Zhang, Xuejiu Wang, Niklas Zender, Robert Sader, C. James Kirkpatrick, Shahram Ghanaati

Published in: Clinical Oral Investigations | Issue 4/2018

Abstract

Objectives

This study examines the permeability and barrier capacity of a sugar cross-linked resorbable collagen membrane ex vivo and in vivo.

Materials and methods

In an ex vivo study, injectable platelet-rich fibrin (i-PRF), a peripheral blood-derived human leukocyte-and-platelet-rich plasma was used to analyze membrane permeability. in vivo subcutaneous implantation in Wistar rats (n = 4 per time point and group) was used to investigate the barrier capacity of the membrane. The induced in vivo cellular reaction was evaluated at 3, 15, and 30 days and compared to sham OP (control) without biomaterial using histological, immunohistochemical, and histomorphometric methods.

Results

Ex vivo, the membrane was impenetrable to leukocytes, platelets, and fibrin from peripheral human blood concentrate (PRF). In vivo, the membrane maintained its structure and remained impervious to cells, connective tissue, and vessels over 30 days. CD-68-positive cell (macrophage) numbers significantly decreased from 3 to 15 days, while from day 15 onwards, the number of multinucleated giant cells (MNGCs) increased significantly. Correspondingly, a rise in implantation bed vascularization from 15 to 30 days was observed. However, no signs of degradation or material breakdown were observed at any time point.

Conclusion

Ex vivo and in vivo results showed material impermeability to cellular infiltration of human and murine cells, which highlights the membrane capacity to serve as a barrier over 30 days. However, whether the induced MNGCs will lead to material degradation or encapsulation over the long term requires further investigation.

Clinical relevance

The data presented are of great clinical interest, as they contribute to the ongoing discussion concerning to what extent an implanted material should be integrated versus serving only as a barrier membrane.

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Title: Sugar-based collagen membrane cross-linking increases barrier capacity of membranes
Authors: Po-ju Chia-Lai
Anna Orlowska
Sarah Al-Maawi
Andre Dias
Yunxin Zhang
Xuejiu Wang
Niklas Zender
Robert Sader
C. James Kirkpatrick
Shahram Ghanaati
Publication date: 01-05-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 4/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2281-1

At a glance: The STEP trials

Springer Medicine

Sugar-based collagen membrane cross-linking increases barrier capacity of membranes

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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