Top

BMC Complementary Medicine and Therapies

Published in:

Open Access 01-12-2018 | Research article

Study on the inhibition of Mfn1 by plant-derived miR5338 mediating the treatment of BPH with rape bee pollen

Authors: Xuan Chen, Ren-zhao Wu, Yong-qiang Zhu, Ze-ming Ren, Ye-ling Tong, Feng Yang, Guan-hai Dai

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

Abstract

Background

Recent studies have found that plant derived microRNA can cross-kingdom regulate the expression of genes in humans and other mammals, thereby resisting diseases. Can exogenous miRNAs cross the blood-prostate barrier and entry prostate then participate in prostate disease treatment?

Methods

Using HiSeq sequencing and RT-qPCR technology, we detected plant miRNAs that enriched in the prostates of rats among the normal group, BPH model group and rape bee pollen group. To forecast the functions of these miRNAs, the psRobot software and TargetFinder software were used to predict their candidate target genes in rat genome. The qRT-PCR technology was used to validate the expression of candidate target genes.

Results

Plant miR5338 was enriched in the posterior lobes of prostate gland of rats fed with rape bee pollen, which was accompanied by the improvement of BPH. Among the predicted target genes of miR5338, Mfn1 was significantly lower in posterior lobes of prostates of rats in the rape bee pollen group than control groups. Further experiments suggested that Mfn1 was highly related to BPH.

Conclusions

These results suggesting that plant-derived miR5338 may involve in treatment of rat BPH through inhibiting Mfn1 in prostate. These results will provide more evidence for plant miRNAs cross-kingdom regulation of animal gene, and will provide preliminary theoretical and experimental basis for development of rape bee pollen into innovative health care product or medicine for the treatment of BPH.

Available only for authorised users

Zhang L, Hou D, Chen X, Li DH, Zhu LY, Zhang YJ, et al. Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA. Cell Res. 2012;22:107–26.CrossRefPubMed

Alvarez-Garcia I, Miska EA. MicroRNA functions in animal development and human disease. Development. 2005;132:4653–62.CrossRefPubMed

Wang K, Li H, Yuan Y, Etheridge A, Zhou Y, Huang D, et al. The complex exogenous RNA spectra in human plasma: an interface with human gut biota? PLoS One. 2012;7:e51009.CrossRefPubMedPubMedCentral

Lukasik A, Zielenkiewicz P. In silico identification of plant miRNAs in mammalian breast milk exosomes – a small step forward? PLoS One. 2014;9:e99963.CrossRefPubMedPubMedCentral

Liang GF, Zhu YZ, Sun B, Shao YH, Jing AH, Wang JH, et al. Assessing the survival of exogenous plant microRNA in mice. Food Sci Nutr. 2014;2:380–8.CrossRefPubMedPubMedCentral

Yang J, Farmer LM, Agyekum AA, Elbaz-Younes I, Hirschi KD. Detection of an abundant plant-based small RNA in healthy consumers. PLoS One. 2015;10:249–56.

Zhou Z, Li X, Liu J, Dong L, Chen Q, Liu JL, et al. Honeysuckle-encoded atypical microRNA2911 directly targets influenza a viruses. Cell Res. 2014;25:39–49.CrossRefPubMedPubMedCentral

Mlotshwa S, Pruss GJ, Macarthur JL, Endres MW, Davis C, Hofseth LJ, et al. A novel chemopreventive strategy based on therapeutic microRNAs produced in plants. Cell Res. 2015;25:521–4.CrossRefPubMedPubMedCentral

Chin AR, Fong MY, Somlo G, Wu J, Swiderski P, Wu XW, et al. Cross-kingdom inhibition of breast cancer growth by plant miR159. Cell Res. 2016;26:217–8.CrossRefPubMedPubMedCentral

10.

Chen X, Dai GH, Ren ZM, Tong YL, Yang F, Zhu YQ. Identification of dietetically absorbed rapeseed (Brassica campestris L.) bee pollen microRNAs in serum of mice. Biomed Res Int. 2016;2016:1–5.

11.

Fulmer BR, Turner TT. A blood-prostate barrier restricts cell and molecular movement across the rat ventral prostate epithelium. J Urol. 2000;163:1591–4.CrossRefPubMed

12.

Cao YL, Meng SX, Chen Y, Feng JX, Gu DD, Yu B, et al. MFN1 structures reveal nucleotide-triggered dimerization critical for mitochondrial fusion. Nature. 2017;542:372–6.CrossRefPubMedPubMedCentral

13.

Choudhary V, Kaddour-Djebbar I, Alaisami R, Kumar MV, Bollag WB. Mitofusin 1 degradation is induced by a disruptor of mitochondrial calcium homeostasis, CGP37157: a role in apoptosis in prostate cancer cells. Int J Oncol. 2014;44:1767–73.CrossRefPubMed

14.

Autret A, Martin SJ. Emerging role for members of the Bcl-2 family in mitochondrial morphogenesis. Mol Cell. 2009;36:355–63.CrossRefPubMed

15.

Qi YB, Yan LM, Yu CT, Guo XY, Zhou X, Hu X, et al. Structures of human mitofusin 1 provide insight into mitochondrial tethering. J Cell Bio. 2016;215:621–9.CrossRef

16.

Philley JV, Kannan A, Qin W, Sauter ER, Ikebe M, Hertweck KL, et al. Complex-I alteration and enhanced mitochondrial fusion are associated with prostate cancer progression. J Cell Physiol. 2016;231:1364–71.CrossRefPubMed

17.

Lou Y, Li R, Liu J, Zhang Y, Zhang X, Jin B, et al. Mitofusin-2 over-expresses and leads to dysregulation of cell cycle and cell invasion in lung adenocarcinoma. Med Oncol. 2015;32:132.CrossRefPubMed

18.

Cipolat S, Martins de Brito O, Dal Zilio B, Scorrano L. OPA1 requires mitofusin 1 to promote mitochondrial fusion. Proc Natl Acad Sci U S A. 2004;101:15927–32.CrossRefPubMedPubMedCentral

19.

Song ZY, Ghochani M, McCaffery JM, Frey TG, Chan DC. Mitofusins and OPA1 mediate sequential steps in mitochondrial membrane fusion. Mol Biol Cell. 2009;20:3525–32.CrossRefPubMedPubMedCentral

20.

Zhang Z, Sun Y, JI L. The dual roles of mitochondrial fusion proteins in mitochondrial fusion and apoptosis. Chinese Bul Life Sci. 2015;27:168–73.

21.

Zhang X, He JQ, Liu LF, Xi JY, Chen BY. Effect of Qianlongtong granules on prostatic cell ultrastructure of experimental prostate hyperplasia rats. Prac Prev Med. 2010;17:639–43.

22.

Saker Z, Tsintsadze O, Jiqia I, Managadze L, Chkhotua A. Importance of apoptosis markers (Mdm2, Bcl-2 and Bax) in benign prostatic prostatic hyperplasia and prostate cancer. Georgian Med News. 2015;249:7–14.

23.

Kowaltowski AJ, Cosso RG, Campos CB, Fiskum G. Effect of Bcl-2 overexpression on mitochondrial structure and function. J Biol Chem. 2002;277:42802–7.CrossRefPubMed

Title: Study on the inhibition of Mfn1 by plant-derived miR5338 mediating the treatment of BPH with rape bee pollen
Authors: Xuan Chen
Ren-zhao Wu
Yong-qiang Zhu
Ze-ming Ren
Ye-ling Tong
Feng Yang
Guan-hai Dai
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-018-2107-y

At a glance: The STEP trials

Springer Medicine

Study on the inhibition of Mfn1 by plant-derived miR5338 mediating the treatment of BPH with rape bee pollen

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2018

Australian integrative oncology services: a mixed-method study exploring the views of cancer survivors

In vitro antileishmanial and cytotoxicity activities of essential oils from Haplophyllum tuberculatum A. Juss leaves, stems and aerial parts

Acacia hydaspica R. Parker ameliorates cisplatin induced oxidative stress, DNA damage and morphological alterations in rat pulmonary tissue

Inhibition of lipopolysaccharide (LPS)-induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (C.D.C.) stem bark, Cryptolepis sanguinolenta (Lindl.) Schltr and Cymbopogon citratus Stapf leaves in raw 264.7 macrophages and U87 glioblastoma cells

Feasibility and acceptability of a proposed trial of acupuncture as an adjunct to lifestyle interventions for weight loss in Polycystic Ovary Syndrome: a qualitative study

Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic syndrome: a randomized clinical trial (PROSIR)