Top

Published in:

01-01-2006 | Original Article

Study of ochratoxin A as an environmental risk that causes renal injury in breast-fed Egyptian infants

Authors: Ahmed M. Hassan, Hussein A. Sheashaa, Mohamed F. Abdel Fattah, Alla Z. Ibrahim, Osama A. Gaber, Mohamed A. Sobh

Published in: Pediatric Nephrology | Issue 1/2006

Abstract

Ochratoxin A (OTA) constitutes a real human threat. Its presence in human milk has previously been reported in different countries. This study is the first Egyptian report on the presence of OTA in both mothers’ milk and infants’ sera, addressing its correlation with infants’ kidney functions, which was not previously addressed in the literature. Fifty healthy breast-lactating mothers and their infants who were exclusively breast-fed for at least 4 months were included. All of them were subjected to a thorough laboratory evaluation including determination of OTA concentration by high-performance liquid chromatography. Thirty-six mothers (72%) and their infants had been contaminated with OTA. Univariate analysis showed that the presence of OTA was associated with significantly higher levels of urinary β2 microglobulin and microalbuminuria. Multivariate logistic regression analysis showed that there was a significant correlation between a higher OTA level in infants’ sera and the degree of microalbuminuria. Mothers and their infants in our locality are exposed to a high OTA contamination rate (72%). To establish the role of OTA in causation of future renal dysfunction for infants, large controlled studies are warranted.

Speijers GJA, Van Egmond HP (1993) Worldwide ochratoxin A levels in food and feeds. In: Creppy EE, Castegnaro M, Dirheimer G (eds) Human ochratoxicosis and its pathologies. Paris Colloque Inserm 231:85–100

Bhatnagar D, Yu J, Ehrlich KC (2002) Toxins of filamentous fungi. Chem Immunol 81:167–206PubMedCrossRef

Wafa EW, Yahya RS, Sobh M, Eraky I, El-Baz M, El-Gayar HAM, Betbeder AM, Creppy EE (1998) Human ochratoxicosis and nephropathy in Egypt: A preliminary study. Hum Exp Toxicol 17:124–129PubMedCrossRef

Skaug MA, Stormer FC, Saugstad OD (1998) Ochratoxin A: a naturally occurring mycotoxin found in human milk samples from Norway. Acta Paediatr 87 (12):1275–1278PubMedCrossRef

Jonsyn FE, Maxwell SM, Hendrickse RG (1995) Ochratoxin A and aflatoxins in breast milk samples from Sierra Leone. Mycopathologia 131(2):121–126PubMedCrossRef

Hadlok RM (1993) Human ochratoxicosis in Germany updating 1993. In: Creppy EE, Castegnaro M, Dirheimer G (eds) Human ochratoxicosis and its pathologies. Paris Colloque Inserm 231:141–145

Micco C, Miraglia M, Brera C, Corneli S, Ambruzzi A (1995) Evaluation of ochratoxin A level in human milk in Italy. Food Addit Contam 12(3):351–354PubMedCrossRef

Turconi G, Guarcello M, Livieri C, Comizzoli S, Maccarini L, Castellazzi AM, Pietri A, Piva G, Roggi C (2004) Evaluation of xenobiotics in human milk and ingestion by the newborn—an epidemiological survey in Lombardy (northern Italy). Eur J Nutr 43(4):191–197PubMedCrossRef

Ferrufino-Guardia EV, Tangni EK, Larondelle Y, Ponchaut S (2000) Transfer of ochratoxin A during lactation: exposure of suckling via the milk of rabbit does fed a naturally-contaminated feed. Food Addit Contam 17(2):167–175PubMedCrossRef

10.

Petkova-Bocharova T, Stoichev II, Chernozemsky IN, Castegnaro M, Pfohl-Leszkowicz A (1998) Formation of DNA adducts in tissues of mouse progeny through transplacental contamination and/or lactation after administration of a single dose of ochratoxin A to the pregnant mother. Environ Mol Mutagen 32(2):155–162PubMedCrossRef

11.

Zimmerli B, Dick R (1995) Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high-performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup: methodology and Swiss data. J Chromatogr B 666:85–89CrossRef

12.

Badria FA (1996) A multidisciplinary study to monitor mycotoxins in Egypt. Egypt J Toxicol 15(3):251–272

13.

Abdelhamid AM (1990) Occurrence of some mycotoxins (aflatoxin, ochratoxin A, citrinin, zearalenone and vomitoxin) in various Egyptian feeds. Arch Tierernahr 40(7):647–664PubMedCrossRef

14.

Monaci L, Palmisano F (2004) Determination of ochratoxin A in foods: state-of-the-art and analytical challenges. Anal Bioanal Chem 378(1):96–103PubMedCrossRef

15.

Castegnaro M, Chernozemsky IN, Hietanen E, Bartsch H (1990) Are mycotoxins risk factors for endemic nephropathy and associated urothelial cancers? Arch Geschwulstforsch 60(4):295–303PubMed

16.

Skaug MA, Helland I, Solvoll K, Saugstad OD (2001) Presence of ochratoxin A in human milk in relation to dietary intake. Food Addit Contam 18(4):321–327PubMedCrossRef

17.

Sauvant C, Holzinger H, Mildenberger S, Gekle M (2005) Exposure to nephrotoxic ochratoxin A enhances collagen secretion in human renal proximal tubular cells. Mol Nutr Food Res 49(1):31–7PubMedCrossRef

18.

Petrik J, Zanic-Grubisic T, Barisic K, Pepeljnjak S, Radic B, Ferencic Z, Cepelak I (2003) Apoptosis and oxidative stress induced by ochratoxin A in rat kidney. Arch Toxicol 77(12):685–693PubMedCrossRef

19.

Hassen W, Abid-Essafi S, Achour A, Guezzah N, Zakhama A, Ellouz F, Creppy EE, Bacha H (2004) Karyomegaly of tubular kidney cells in human chronic interstitial nephropathy in Tunisia: respective role of Ochratoxin A and possible genetic predisposition. Hum Exp Toxicol 23(7):339–346PubMedCrossRef

20.

Abid S, Hassen W, Achour A, Skhiri H, Maaroufi K, Ellouz F, Creppy E, Bacha H (2003) Ochratoxin A and human chronic nephropathy in Tunisia: is the situation endemic? Hum Exp Toxicol 22(2):77–84PubMedCrossRef

21.

Hsieh MF, Chiu HY, Lin-Tan DT, Lin JL (2004) Does human ochratoxin A aggravate proteinuria in patients with chronic renal disease? Ren Fail 26(3):311–316PubMedCrossRef

22.

Vrabcheva T, Petkova-Bocharova T, Grosso F, Nikolov I, Chernozemsky IN, Castegnaro M, Dragacci S (2004) Analysis of ochratoxin A in foods consumed by inhabitants from an area with Balkan endemic nephropathy: a 1-month follow-up study. J Agric Food Chem 52(8):2404–2410PubMedCrossRef

23.

Hassen W, Abid S, Achour A, Creppy E, Bacha H (2004) Ochratoxin A and beta2-microglobulinuria in healthy individuals and in chronic interstitial nephropathy patients in the centre of Tunisia: a hot spot of Ochratoxin A exposure. Toxicology 199:185–193PubMedCrossRef

24.

(2002) Assessment of dietary intake of Ochratoxin by the population of EU Member States. Report of experts participating in Task 3.2.7 January 2002

Title: Study of ochratoxin A as an environmental risk that causes renal injury in breast-fed Egyptian infants
Authors: Ahmed M. Hassan
Hussein A. Sheashaa
Mohamed F. Abdel Fattah
Alla Z. Ibrahim
Osama A. Gaber
Mohamed A. Sobh
Publication date: 01-01-2006
Publisher: Springer-Verlag
Published in: Pediatric Nephrology / Issue 1/2006
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-005-2033-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Study of ochratoxin A as an environmental risk that causes renal injury in breast-fed Egyptian infants

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2006

The efficiency of intraperitoneal high-dose immunoglobulin in experimental nephrotic syndrome

Timed-urine collections for renal clearance studies

Steroid elimination is coming of age

IgA deficiency and membranous glomerulonephritis

Hemolytic uremic syndrome associated withEntamoeba histolytica intestinal infection

Clinical course of prenatally detected primary vesicoureteral reflux