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BMC Oral Health
Published in: BMC Oral Health 1/2021

Open Access 01-12-2021 | Streptococci | Research article

Analysis of oral microbial dysbiosis associated with early childhood caries

Authors: Hui Zheng, Tengfei Xie, Shaokai Li, Xiaotong Qiao, Youguang Lu, Yan Feng

Published in: BMC Oral Health | Issue 1/2021

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Abstract

Background

"Core microbes" play a key role in the development of caries and lead to microbial disorders. Our goal was to detect the core microbes associated with the microbiota imbalance in early childhood caries (ECC).

Methods

Fifteen caries-free children and fifteen high-caries (DMFT ≥ 10) children aged 4–6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from samples of plaque in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms.

Results

There were statistically significant differences between the groups regarding the relative abundance of Streptococcus mutans (Wilcoxon rank-sum test, P < 0.05). No significant difference was found between the groups regarding other species or functional genes.

Conclusion

S. mutans, together with other pathogens, may play a prominent role and act as "core microbes" in the occurrence and development of early childhood caries.
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Metadata
Title
Analysis of oral microbial dysbiosis associated with early childhood caries
Authors
Hui Zheng
Tengfei Xie
Shaokai Li
Xiaotong Qiao
Youguang Lu
Yan Feng
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2021
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-021-01543-x

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