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Published in:

01-01-2005 | Original Article

Steroid effects on osteogenesis through mesenchymal cell gene expression

Authors: Xudong Li, Li Jin, Quanjun Cui, Gwo-Jaw Wang, Gary Balian

Published in: Osteoporosis International | Issue 1/2005

Abstract

We have studied the mechanism of steroid-induced osteonecrosis by examining the effect of dexamethasone on a multipotential cell line, D1, which is derived from bone marrow and is capable of differentiating into either the osteoblast or the adipocyte lineage. The expression of bone cell and fat cell transcription factors Cbfa1/Runx2 and PPARγ2, were determined. Osteocalcin promoter activity was measured by co-transfecting the cells with the phOC-luc and pSV β-Gal plasmids. Dexamethasone increased PPARγ2 gene expression 2-fold, while Cbfa1/Runx2 gene expression and osteocalcin promoter activity decreased by 50–60%, and VEGF protein, measured by ELISA, decreased by 55%. These changes indicate enhanced adipogenesis and decreased osteogenesis by mesenchymal cells in vitro, together with a decrease in VEGF, a potent angiogeneic factor, suggesting that dexamethasone may shunt uncommitted osteoprogenitor cells in marrow from osteoblastic differentiation into the adipocytic pathway, leading to diminished vascularization and eventual osteonecrosis.

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Title: Steroid effects on osteogenesis through mesenchymal cell gene expression
Authors: Xudong Li
Li Jin
Quanjun Cui
Gwo-Jaw Wang
Gary Balian
Publication date: 01-01-2005
Publisher: Springer-Verlag
Published in: Osteoporosis International / Issue 1/2005
Print ISSN: 0937-941X
Electronic ISSN: 1433-2965
DOI: https://doi.org/10.1007/s00198-004-1649-7

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Springer Medicine

Steroid effects on osteogenesis through mesenchymal cell gene expression

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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