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European Journal of Orthopaedic Surgery & Traumatology

Published in:

01-02-2013 | Original Article

Stem cells derived from osteoarthritic knee mesenchymal tissues: a pilot study

Authors: Luminita Simion Labusca, Paul Botez, Florin Zugun Eloae, Kaveh Mashayekhi

Published in: European Journal of Orthopaedic Surgery & Traumatology | Issue 2/2013

Abstract

Regenerative medicine is a promising approach for addressing musculoskeletal disorders. Successful implementation of regenerative therapies is based upon existence of reliable, easy accessible cell sources. Mesenchymal tissues removed during total knee replacement (TKR) were investigated as a potential autologous stem cell source. Materials and methods Samples were collected from patients undergoing primary TKR mononuclear cells from adipose and synovial tissue; subchondral trabecular bone and osteoarthritic cartilage were isolated and assessed in terms of mesenchymal stem cells (MSC) content. Results MSCs obtained from all the investigated tissue types and from all donors showed proliferative, differentiation and surface markers characteristic of stemness. Important number of MSCs could be obtained in the first passage (P0). Mesenchymal tissues removed during TJR can qualitatively and quantitatively function as autologous MSC sources to be considered for regenerative therapies.

Mason C, Dunnill P (2008) A brief definition of regenerative medicine. Regen Med 3(1):1–5PubMedCrossRef

Foundations of regenerative medicine, Atala A, Lanza R, Thompson J, Nerem R. (Editors) first edition 2010, Elsevier CA (US), MA (US) UK, 1–5

Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L (1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331:889–895PubMedCrossRef

Bourin Ph, Luc S, Planat-Benard V, Jerome R, Bura-Rivierre A, Casteilla L (2010) Culture and use of mesenchymal stromal cells in phase I and II clinical trials. Stem Cells Int, art ID 503593, p 8

Murphy JM, Fink DJ, Hunziker EB, Barry FP (2003) Stem cell therapy in a caprine model of osteoarthritis. Arthr Rheum 48(12):3464–3467CrossRef

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147PubMedCrossRef

Caplan AI, Bruder SP (2001) Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends Mol Med 6:259–264CrossRef

Bourin Ph, Luc S, Planat-Benard V, Jerome R, Bura-Rivierre A, Casteilla L (2010) Culture and use of mesenchymal stromal cells in phase I and II clinical trials. Stem Cells Int, art ID 503593, p 8

Li WJ, Tuli R, Huang X, Laquerriere P, Tuan RS (2005) Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials 26(25):5158–5166PubMedCrossRef

10.

Sakaguchi Y, Sekiya I, Yagishita K, Muneta T (2005) Comparison of human stem cells derived from various mesenchymal tissues superiority of synovium as a cell source. Arthr Rheum 52(8):2521–2529CrossRef

11.

Koelling S, Kruger J, Irmer M, Path JR, Sadovski B, Miro X, Miosgue N (2009) Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis. Cell Stem Cell 204:324–335CrossRef

12.

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7(2):211–228PubMedCrossRef

13.

Oedayrajsingh-Varma MJ, van Ham SM, Knippenberg M, Helder MN, Klein-Nulend J, Schouten TE, Ritt MJ, van Milligen FJM (2006) Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy 8(2):166–177PubMedCrossRef

14.

Dumond H, Presle N, Terlain B, Mainard D, Loeuille D, Netter P, Pottie P (2003) Evidence for a key role of leptin in osteoarthritis. Arthr Rheum 48(11):3118–3129CrossRef

15.

De Bari C, Dell’Accio F, Tylzanowski P, Luyten FP (2001) Multipotent mesenchymal stem cells from adult human synovial membrane. Arthr Rheum 44(8):1928–1942CrossRef

16.

Sakaguki Y, Sekyia I, Yagishita K, Ichinose S, Shinomyia K, Muneta T (2004) Suspended cells from trabecular bone by collagenases digestion become virtually identical to mesenchymals stem cells obtained from bone marrow aspirates. Blood 104(9):2728–2735CrossRef

17.

Tuli R, Seghatoleslami MR, Tuli S, Wang ML, Hozack WJ, Manner PA, Danielson KG, Tuan RS (2003) A simple, high-yield method for obtaining multipotential mesenchymal progenitor cells from trabecular bone. Mol Biotechnol 23(1):37–49PubMedCrossRef

18.

Stolzing A, Jones E, McGonagle D, Scutt A (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies. Mech Ageing Dev 129(3):163–173PubMedCrossRef

19.

Coipeau P, Rosset P, Langonne A, Gaillard J, Delorme B, Rico A, Domenech J, Charbord P, Sensebe L (2009) Impaired differentiation potential of human trabecular bone mesenchymal stromal cells from elderly patients. Cytotherapy 11(5):584–589PubMedCrossRef

20.

Castro-Malaspina H, Ebell W, Wang S (1984) Human bone marrow fibroblast colony-forming units (CFU-F). Prog Clin Biol Res 154:209–236PubMed

21.

Nimura A, Muneta T, Otabe K, Koga H, Ju YJ, Mochizuki T, Suzuki K, Sekiya I (2009) Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums. BMC Musculoskelet Disord 14(11):208

22.

Wakitani S, Nawata M, Tensho K, Okabe T, Machida H, Ohgushi H (2007) Repair of articular cartilage defects in the patella-femoral joint with autologous bone marrow mesenchymal cell transplantation: three case reports involving nine defects in five knees. J Tissue Eng Regen Med 1:74–81PubMedCrossRef

23.

Haleem A, El-Singergy A, Sabry D et al (2010) The clinical use of human culture-expanded autologous bone marrow mesenchymal stem cells transplanted on platelet-rich fibrin glue in the treatment of articular cartilage defects: a pilot study and preliminary results. Cartilage 1(4):253–261PubMedCrossRef

24.

Ohgushi H, Kitamura S, Kotobuki N et al (2004) Clinical application of bone marrow mesenchymal stem cells for hard tissue repair. Yonsei Med J 45(Suppl):61–67PubMed

Title: Stem cells derived from osteoarthritic knee mesenchymal tissues: a pilot study
Authors: Luminita Simion Labusca
Paul Botez
Florin Zugun Eloae
Kaveh Mashayekhi
Publication date: 01-02-2013
Publisher: Springer-Verlag
Published in: European Journal of Orthopaedic Surgery & Traumatology / Issue 2/2013
Print ISSN: 1633-8065
Electronic ISSN: 1432-1068
DOI: https://doi.org/10.1007/s00590-012-0949-4

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Stem cells derived from osteoarthritic knee mesenchymal tissues: a pilot study

Abstract

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