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01-02-2017 | 2016 SSAT Poster Presentation

Sponge Sampling with Fluorescent In Situ Hybridization as a Screening Tool for the Early Detection of Esophageal Cancer

Authors: Kelly R. Haisley, MD, James P. Dolan, MD, MCR, Susan B. Olson, PhD, Sergio A. Toledo-Valdovinos, MD, Kyle D. Hart, MS, Gene Bakis, MD, Brintha K. Enestvedt, MD, John G. Hunter, MD

Published in: Journal of Gastrointestinal Surgery | Issue 2/2017

Abstract

Introduction

Sponge cytology is a novel screening tool for esophageal cancer but has been unable to be validated for widespread use. Our aim was to apply fluorescent in situ hybridization to sponge cytology samples in order to evaluate the safety and efficacy of this modality in screening for esophageal cancer.

Materials and Methods

At a single, multidisciplinary, NCI-designated cancer center, patients completed sponge cytology sampling prior to upper endoscopy. Samples were analyzed by p53 fluorescent in situ hybridization, and results were compared to the endoscopic diagnosis.

Results

Fifty patients were enrolled (96 % Caucasian, 68 % male, median age of 67). All patients successfully swallowed the capsule. No complications (string breakage, bleeding, mucosal injury) occurred. Endoscopy revealed that 38 % had normal esophageal mucosa and 62 % had an esophageal mucosal abnormality. In total, six samples demonstrated p53 loss (94 % specificity for any abnormality). The sensitivity of the p53 fluorescent in situ hybridization probe was13.3 % for any abnormality, 10 % for intestinal metaplasia, and 0 % for dysplasia or esophageal cancer.

Discussion

Esophageal sponge cytology is a promising, safe, and tolerable method for collecting esophageal cell samples. However, our data suggest that p53 fluorescent in situ hybridization does not improve the sensitivity for detecting cancer in these samples.

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Title: Sponge Sampling with Fluorescent In Situ Hybridization as a Screening Tool for the Early Detection of Esophageal Cancer
Authors: Kelly R. Haisley, MD
James P. Dolan, MD, MCR
Susan B. Olson, PhD
Sergio A. Toledo-Valdovinos, MD
Kyle D. Hart, MS
Gene Bakis, MD
Brintha K. Enestvedt, MD
John G. Hunter, MD
Publication date: 01-02-2017
Publisher: Springer US
Published in: Journal of Gastrointestinal Surgery / Issue 2/2017
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-016-3239-3

At a glance: The STEP trials

Springer Medicine

Sponge Sampling with Fluorescent In Situ Hybridization as a Screening Tool for the Early Detection of Esophageal Cancer

Abstract

Introduction

Materials and Methods

Results

Discussion

At a glance: The STEP trials

Springer Medicine

Abstract

Introduction

Materials and Methods

Results

Discussion

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