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Open Access 01-12-2013 | Research

Splice variants of zinc finger protein 695 mRNA associated to ovarian cancer

Authors: Sergio Juárez-Méndez, Alejandro Zentella-Dehesa, Vanessa Villegas-Ruíz, Oscar Alberto Pérez-González, Mauricio Salcedo, Ricardo López-Romero, Edgar Román-Basaure, Minerva Lazos-Ochoa, Víctor Edén Montes de Oca-Fuentes, Guelaguetza Vázquez-Ortiz, José Moreno

Published in: Journal of Ovarian Research | Issue 1/2013

Abstract

Background

Studies of alternative mRNA splicing (AS) in health and disease have yet to yield the complete picture of protein diversity and its role in physiology and pathology. Some forms of cancer appear to be associated to certain alternative mRNA splice variants, but their role in the cancer development and outcome is unclear.

Methods

We examined AS profiles by means of whole genome exon expression microarrays (Affymetrix GeneChip 1.0) in ovarian tumors and ovarian cancer-derived cell lines, compared to healthy ovarian tissue. Alternatively spliced genes expressed predominantly in ovarian tumors and cell lines were confirmed by RT-PCR.

Results

Among several significantly overexpressed AS genes in malignant ovarian tumors and ovarian cancer cell lines, the most significant one was that of the zinc finger protein ZNF695, with two previously unknown mRNA splice variants identified in ovarian tumors and cell lines. The identity of ZNF695 AS variants was confirmed by cloning and sequencing of the amplicons obtained from ovarian cancer tissue and cell lines.

Conclusions

Alternative ZNF695 mRNA splicing could be a marker of ovarian cancer with possible implications on its pathogenesis.

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Title: Splice variants of zinc finger protein 695 mRNA associated to ovarian cancer
Authors: Sergio Juárez-Méndez
Alejandro Zentella-Dehesa
Vanessa Villegas-Ruíz
Oscar Alberto Pérez-González
Mauricio Salcedo
Ricardo López-Romero
Edgar Román-Basaure
Minerva Lazos-Ochoa
Víctor Edén Montes de Oca-Fuentes
Guelaguetza Vázquez-Ortiz
José Moreno
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2013
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/1757-2215-6-61

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Splice variants of zinc finger protein 695 mRNA associated to ovarian cancer

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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