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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-01-2009 | Basic Science

Splenic CD8⁺ T cells secrete TGF-β1 to exert suppression in mice with anterior chamber-associated immune deviation

Authors: Liqiong Jiang, Hao He, Peizeng Yang, Xiaomin Lin, Hongyan Zhou, Xiangkun Huang, Aize Kijlstra

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 1/2009

Abstract

Background

CD8⁺ regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8⁺ T cells in ACAID remain only poorly understood. TGF-β1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-β1 by CD8⁺ T cells in ACAID, and whether CD8⁺ T cells exert suppression through TGF-β1, is unknown.

Methods

The suppressive effect of CD8⁺ T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-β1 by CD8⁺ T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-β1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8⁺ T cells.

Results

CD8⁺ T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8⁺ T cells secreted TGF-β1, and their suppression could partially be blocked by anti-TGF-β1 antibodies.

Conclusions

Our study confirms that CD8⁺ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-β1.

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Title: Splenic CD8+ T cells secrete TGF-β1 to exert suppression in mice with anterior chamber-associated immune deviation
Authors: Liqiong Jiang
Hao He
Peizeng Yang
Xiaomin Lin
Hongyan Zhou
Xiangkun Huang
Aize Kijlstra
Publication date: 01-01-2009
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 1/2009
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-008-0947-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Splenic CD8⁺ T cells secrete TGF-β1 to exert suppression in mice with anterior chamber-associated immune deviation

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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