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Journal of Assisted Reproduction and Genetics

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01-06-2012 | Technical Innovations

Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

Authors: Varshini Jayaraman, Dinesh Upadhya, Pratap Kumar Narayan, Satish Kumar Adiga

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2012

Abstract

Purpose

DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage.

Methods

A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment.

Results

The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable.

Conclusions

Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.

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Title: Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage
Authors: Varshini Jayaraman
Dinesh Upadhya
Pratap Kumar Narayan
Satish Kumar Adiga
Publication date: 01-06-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9742-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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