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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2003

Open Access 01-12-2003 | Review

Specific genetic modifications of domestic animals by gene targeting and animal cloning

Authors: Bin Wang, Jiangfeng Zhou

Published in: Reproductive Biology and Endocrinology | Issue 1/2003

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Abstract

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large livestock species until the successful creation of the first mammalian clone "Dolly" the sheep. The combination of the technologies for gene targeting of somatic cells with those of animal cloning made it possible to introduce specific genetic mutations into domestic animals. In this review, the principles of gene targeting in somatic cells and the challenges of nuclear transfer using gene-targeted cells are discussed. The relevance of gene targeting in domestic animals for applications in bio-medicine and agriculture are also examined.
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Metadata
Title
Specific genetic modifications of domestic animals by gene targeting and animal cloning
Authors
Bin Wang
Jiangfeng Zhou
Publication date
01-12-2003
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2003
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-1-103

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