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Tumor Biology
Published in: Tumor Biology 5/2016

01-05-2016 | Original Article

Sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway

Authors: Yue-Can Zeng, Rui Xing, Jing Zeng, Ming Xue, Feng Chi, Yan Xin, Guo-Liang Fan, Hong-Mei Wang, Qiong-Yu Duan, Yu-Nan Sun, Nan Niu, Rong Wu

Published in: Tumor Biology | Issue 5/2016

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Abstract

The purpose of this study was to evaluate the radiation-enhancing effect of sodium glycididazole, and the corresponding mechanisms of action on laryngeal cancer cells. Two laryngeal cancer cell lines (Hep-2 and UT-SCC-19A) were irradiated with X-rays in the presence or absence of sodium glycididazole. Cell survival, DNA damage and repair, cell apoptosis, cell cycle distribution, expression of proteins related to cell cycle checkpoint, and apoptosis were measured. Significantly increased DNA damages, decreased cells in the G1 phase, arrested cells at G2/M phase, decreased DNA repair protein XRCC1 foci formation, and enhanced cell apoptosis were observed in laryngeal cell lines treated by sodium glycididazole combined with irradiation compared with the irradiation alone. The combined treatment downregulated the protein expressions of ataxia–telangiectasia mutated (ATM), p-ATM, CHK2, and P53 but upregulated the protein expressions of MDM2 and Cdk2. This study indicates that sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway in vitro and in vivo.
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Metadata
Title
Sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway
Authors
Yue-Can Zeng
Rui Xing
Jing Zeng
Ming Xue
Feng Chi
Yan Xin
Guo-Liang Fan
Hong-Mei Wang
Qiong-Yu Duan
Yu-Nan Sun
Nan Niu
Rong Wu
Publication date
01-05-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 5/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-4278-1

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