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01-10-2012 | Original Paper

Sodium butyrate increases the effect of the photodynamic therapy: a mechanism that involves modulation of gene expression and differentiation in astrocytoma cells

Authors: José Bueno-Carrazco, Violeta Castro-Leyva, Fanny García-Gomez, Mario Solís-Paredes, Eva Ramon-Gallegos, Alfredo Cruz-Orea, Pilar Eguía-Aguilar, Francisco Arenas-Huertero

Published in: Child's Nervous System | Issue 10/2012

Abstract

Objectives

In order to evaluate the improvement of the photodynamic therapy (PDT) due to sodium butyrate (NaBu), its effectiveness in U373-MG and D54-MG astrocytoma cell lines was evaluated.

Methods

Cells were exposed to delta-aminolevulinic acid (δ-ALA) as a precursor to endogenous photosensitizer protoporphyrin IX (PpIX). In both astrocytoma cells, an important increase by ALA was observed in uroporphyrinogen synthetase gene expression: 1.8- and 52-fold for D54-MG and U373-MG cells, respectively. After irradiation, they showed 16.67 and 28.9 % of mortality in U373-MG and D54-MG, respectively. These mortalities increased to 70.62 and 96.7 % when U373-MG and D54-MG cells, respectively, were exposed 24 h to 8 mM NaBu, before to PpIX induction. NaBu induced expression of caspase-3, caspase-9, and Bcl-2 and increased Bax in U373-MG cells. ALA-induced morphological changes are compatible to differentiation.

Conclusions

Genes and differentiation induced mainly by NaBu improve cell death performed by PDT in astrocytoma cells. These facts prove the synergistic effect of NaBu on cytotoxic damage induced by PDT.

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Title: Sodium butyrate increases the effect of the photodynamic therapy: a mechanism that involves modulation of gene expression and differentiation in astrocytoma cells
Authors: José Bueno-Carrazco
Violeta Castro-Leyva
Fanny García-Gomez
Mario Solís-Paredes
Eva Ramon-Gallegos
Alfredo Cruz-Orea
Pilar Eguía-Aguilar
Francisco Arenas-Huertero
Publication date: 01-10-2012
Publisher: Springer-Verlag
Published in: Child's Nervous System / Issue 10/2012
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI: https://doi.org/10.1007/s00381-012-1828-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Sodium butyrate increases the effect of the photodynamic therapy: a mechanism that involves modulation of gene expression and differentiation in astrocytoma cells

Abstract

Objectives

Methods

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Methods

Conclusions

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