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Pediatric Surgery International

Published in:

01-02-2012 | Original Article

Smooth muscle proteins from Hirschsprung’s disease facilitates stem cell differentiation

Authors: Cornelia Irene Hagl, Sabine Heumüller, Markus Klotz, Ulrike Subotic, Lucas Wessel, Karl-Herbert Schäfer

Published in: Pediatric Surgery International | Issue 2/2012

Abstract

Background and aims

The transplantation of neural crest derived stem cells (NCSC’s) is a potent alternative for the treatment of Hirschsprung’s disease (HSCR). Cells to be transplanted should find an appropriate microenvironment to survive and differentiate. To investigate the quality of this microenvironment, effects of HSCR-smooth-muscle-protein extracts upon NCSC’s were studied in vitro.

Methods

Postnatal human gut from children undergoing colonic resection due to HSCR was divided in segments. Smooth muscle was dissected and homogenized. Glial-cell-line-derived-neurotrophic-factor (GDNF) concentration was measured in the homogenates from the individual segment using ELISA. NCSC’s were exposed to protein extracts derived from ganglionic and aganglionic HSCR segments, and their effect upon neurite outgrowth, survival and branching was evaluated.

Results

The amount of the factors varied considerably between the proximal and distal segments, and also from patient to patient. While extracts from proximal segments tended to have more prominent effects, all HSCR-muscle-protein extracts increased neuronal survival and network formation.

Conclusion

Muscle protein from aganglionic bowel supports the survival and outgrowth of NCSC’s and is so an appropriate target for neural stem cell treatment.

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Title: Smooth muscle proteins from Hirschsprung’s disease facilitates stem cell differentiation
Authors: Cornelia Irene Hagl
Sabine Heumüller
Markus Klotz
Ulrike Subotic
Lucas Wessel
Karl-Herbert Schäfer
Publication date: 01-02-2012
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 2/2012
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-011-3010-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Smooth muscle proteins from Hirschsprung’s disease facilitates stem cell differentiation

Abstract

Background and aims

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background and aims

Methods

Results

Conclusion

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