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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

SKA1 overexpression is associated with poor prognosis in hepatocellular carcinoma

Authors: Yibing Chen, Jingjing Zhao, Zhihui Jiao, Weiwei Wang, Dandan Wang, Xiaohe Yu, Zhiyong Shi, Naijian Ge, Qiuzhong Pan, Jianchuan Xia, Wancheng Niu, Ruihua Zhao, Xiaofei Zhang, Wei Du

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

SKA1, an important mitosis protein, has been indicated in the initiation and progression of several malignancies. However, its clinical significance in hepatocellular carcinoma (HCC) remain to be elucidated.

Methods

mRNA expression of SKA1 was examined in 126 HCC and paired non-neoplastic tissues using real-time PCR and validated in The Cancer Genome Atlas (TCGA) database. SKA1 protein expression was detected using immunohistochemistry in the 126 HCC tissues and its associations with clinicopathological parameters and prognosis were analyzed. Hierarchical cluster analysis and gene set enrichment analysis (GSEA) were performed in selected Gene Expression Omnibus data sets.

Results

SKA1 mRNA expression was significantly elevated in HCC tissues from both local hospital and TCGA database. Immunohistochemistry revealed that increased SKA1 expression was present in 65 of the 126 cases and was significantly associated with higher serum alpha-fetoprotein concentration, larger tumor size and higher TNM stage. Patients with positive SKA1 expression showed significantly worse overall and relapse-free survival. Multivariate Cox regression analysis revealed that SKA1 was an independent predictor of patient prognosis. Gene expression profiling analysis of public data showed that high-SKA1 expression HCC tissues had similar gene expression profiles with fetal liver tissues. Moreover, GSEA showed that genes up-regulated in high SKA1 HCC subgroup were significantly enriched in cell cycle pathway, while genes down-regulated were significantly enriched in apoptosis pathway.

Conclusions

Our findings indicate that the oncofetal gene SKA1 might be involved in the progression of the HCC and could serve as a prognostic marker for HCC.
Appendix
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Metadata
Title
SKA1 overexpression is associated with poor prognosis in hepatocellular carcinoma
Authors
Yibing Chen
Jingjing Zhao
Zhihui Jiao
Weiwei Wang
Dandan Wang
Xiaohe Yu
Zhiyong Shi
Naijian Ge
Qiuzhong Pan
Jianchuan Xia
Wancheng Niu
Ruihua Zhao
Xiaofei Zhang
Wei Du
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-5119-6

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