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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 3/2011

01-06-2011 | Research Article

Simultaneous Dual-tracer PET Imaging of the Rat Brain and its Application in the Study of Cerebral Ischemia

Authors: Francisca P. Figueiras, Xavier Jiménez, Deborah Pareto, Vanessa Gómez, Jordi Llop, Raul Herance, Santiago Rojas, Juan D. Gispert

Published in: Molecular Imaging and Biology | Issue 3/2011

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Abstract

Purpose

This study evaluates the performance of simultaneous dual-tracer technique (SDTT) in static positron emission tomography (PET) studies using 2-deoxy-2-[18F]fluoro-D-glucose and [13N]ammonium as radiotracers.

Procedures

The effects of applying SDTT either to the reconstructed image or directly to the sinogram, different rebinning algorithms, total acquisition time, and frame duration were investigated; first, using a specific phantom and later using an in vivo application of the study of cerebral ischemia.

Results

The best results were obtained using the image method with single-slice rebinning and a total acquisition time of at least 20 min. Frame duration did not affect SDTT performance. The method was also applied in rats with transient cerebral ischemia to simultaneously study cerebral blood flow and cerebral glucose metabolism.

Conclusion

The results encourage the use of SDTT as it has very good potential for examining two different biological processes at the same time utilising rodent PET scanners.
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Metadata
Title
Simultaneous Dual-tracer PET Imaging of the Rat Brain and its Application in the Study of Cerebral Ischemia
Authors
Francisca P. Figueiras
Xavier Jiménez
Deborah Pareto
Vanessa Gómez
Jordi Llop
Raul Herance
Santiago Rojas
Juan D. Gispert
Publication date
01-06-2011
Publisher
Springer-Verlag
Published in
Molecular Imaging and Biology / Issue 3/2011
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-010-0370-5

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