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01-01-2012 | Physiology Research

Sexual Dimorphism in Clock Genes Expression in Human Adipose Tissue

Authors: P. Gómez-Abellán, J. A. Madrid, J. A. Luján, M. D. Frutos, R. González, O. Martínez-Augustín, F. Sánchez de Medina, J. M. Ordovás, M. Garaulet

Published in: Obesity Surgery | Issue 1/2012

Abstract

Background

This study was carried out to investigate whether sex-related differences exist in the adipocyte expression of clock genes from subcutaneous abdominal and visceral fat depots in severely obese patients.

Methods

We investigated 16 morbidly obese patients, eight men and eight women (mean age 45 ± 20 years; mean BMI 46 ± 6 kg/m²), undergoing laparoscopic gastric bypass surgery. Biopsies were taken as paired samples [subcutaneous and visceral adipose tissue (AT)] at the beginning of the surgical process at 11:00 h in the morning. Metabolic syndrome features such as waist circumference, plasma glucose, triglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were also studied. The expression of clock genes (PER2, BMAL1, and CRY1) was measured by quantitative real-time PCR, Western blot, and immunohistochemical analysis.

Results

Gene expression was significantly higher in women than in men for the three genes studied in both ATs (P < 0.05). In visceral fat, these differences were more marked. (P < 0.001). Western blot analysis partially confirmed these results since statistical differences were observed for PER2 in both ATs and for CRY1 in subcutaneous adipose tissue. There were no differences in BMAL1 protein expression. Interestingly, clock gene expression level was correlated with LDL-C and HDL-C (P < 0.05). Moreover, we found significant associations with body fat mass in women and with age in men.

Conclusions

Clock genes expression is sex dependent in human adipose tissue from morbidly obese subjects and correlates to a decreased in metabolic syndrome-related traits. These preliminary results make necessary to go deep into the knowledge of the molecular basis of the sexual dimorphism in chronobiology.

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Title: Sexual Dimorphism in Clock Genes Expression in Human Adipose Tissue
Authors: P. Gómez-Abellán
J. A. Madrid
J. A. Luján
M. D. Frutos
R. González
O. Martínez-Augustín
F. Sánchez de Medina
J. M. Ordovás
M. Garaulet
Publication date: 01-01-2012
Publisher: Springer-Verlag
Published in: Obesity Surgery / Issue 1/2012
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI: https://doi.org/10.1007/s11695-011-0539-2

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Springer Medicine

Sexual Dimorphism in Clock Genes Expression in Human Adipose Tissue

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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