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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2010

Open Access 01-12-2010 | Research article

Seroepidemiology of pandemic influenza A (H1N1) 2009 virus infections in Pune, India

Authors: Babasaheb V Tandale, Shailesh D Pawar, Yogesh K Gurav, Mandeep S Chadha, Santosh S Koratkar, Vijay N Shelke, Akhilesh C Mishra

Published in: BMC Infectious Diseases | Issue 1/2010

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Abstract

Background

In India, Pune was one of the badly affected cities during the influenza A (H1N1) 2009 pandemic. We undertook serosurveys among the risk groups and general population to determine the extent of pandemic influenza A (H1N1) 2009 virus infections.

Methods

Pre-pandemic sera from the archives, collected during January 2005 to March 2009, were assayed for the determination of baseline seropositivity. Serosurveys were undertaken among the risk groups such as hospital staff, general practitioners, school children and staff and general population between 15th August and 11th December 2009. In addition, the PCR-confirmed pandemic influenza A (H1N1) 2009 cases and their household contacts were also investigated. Haemagglutination-inhibition (HI) assays were performed using turkey red blood cells employing standard protocols. A titre of ≥1:40 was considered seropositive.

Results

Only 2 (0.9%) of the 222 pre-pandemic sera were positive. The test-retest reliability of HI assay in 101 sera was 98% for pandemic H1N1, 93.1% for seasonal H1N1 and 94% for seasonal H3N2. The sera from 48 (73.8%) of 65 PCR-confirmed pandemic H1N1 cases in 2009 were positive. Seropositivity among general practitioners increased from 4.9% in August to 9.4% in November and 15.1% in December. Among hospital staff, seropositivity increased from 2.8% in August to 12% in November. Seropositivity among the schools increased from 2% in August to 10.7% in September. The seropositivity among students (25%) was higher than the school staff in September. In a general population survey in October 2009, seropositivity was higher in children (9.1%) than adults (4.3%). The 15-19 years age group showed the highest seropositivity of 20.3%. Seropositivity of seasonal H3N2 (55.3%) and H1N1 (26.4%) was higher than pandemic H1N1 (5.7%) (n = 2328). In households of 74 PCR-confirmed pandemic H1N1 cases, 25.6% contacts were seropositive. Almost 90% pandemic H1N1 infections were asymptomatic or mild. Considering a titre cut off of 1:10, seropositivity was 1.5-3 times as compared to 1:40.

Conclusions

Pandemic influenza A (H1N1) 2009 virus infection was widespread in all sections of community. However, infection was significantly higher in school children and general practitioners. Hospital staff had the lowest infections suggesting the efficacy of infection-control measures.
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Literature
1.
2.
Mishra AC, Chadha MS, Choudhary ML, Potdar VA: Pandemic influenza (H1N1) 2009 is associated with severe disease in India. PLoS One. 2010, 5 (5): e10540-10.1371/journal.pone.0010540.CrossRefPubMedPubMedCentral
3.
World Health Organization: Human infection with new influenza A (H1N1) virus: clinical observations from Mexico and other affected countries, May 2009. Wkly Epidemiol Rec. 2009, 84: 185-189.
4.
Lipsitch M, Riley S, Ferguson NM: Managing and reducing uncertainty in an emerging influenza pandemic. N Engl J Med. 2009, 361: 112-115. 10.1056/NEJMp0904380.CrossRefPubMedPubMedCentral
5.
Hancock K, Veguilla V, Lu X, Zhong W, Butler EN, Sun H, Liu F, Dong L, DeVos JR, Gargiullo PM, Brammer TL, Cox NJ, Tumpey TM, Katz JM: Cross-reactive antibody responses to the 2009 pandemic H1N1 influenza virus. N Engl J Med. 2009, 361: 1945-1952. 10.1056/NEJMoa0906453.CrossRefPubMed
6.
7.
Chen H, Wang Y, Liu W, Zhang J, Dong B, Fan X, de Jong MD, Farrar J, Riley S, Smith GJ, Guan Y: Serologic survey of pandemic (H1N1) 2009 virus, Guangxi Province, China. Emerg Infect Dis. 2009, 15: 1849-1850.CrossRefPubMedPubMedCentral
8.
Ikonen N, Strengell M, Kinnunen L, Osterlund P, Pirhonen J, Broman M, Davidkin I, Ziegler T, Julkunen I: High frequency of cross-reacting antibodies against 2009 pandemic influenza A(H1N1) virus among the elderly in Finland. Euro surveill. 2010, 15: pii:19478-
9.
Miller E, Hoschler K, Hardelid P, Stanford E, Andrews N, Zambon M: Incidence of 2009 pandemic influenza A H1N1 infection in England: a cross-sectional serological study. Lancet. 2010, 375: 1100-1108. 10.1016/S0140-6736(09)62126-7.CrossRefPubMed
10.
Ross T, Zimmer S, Burke D, Crevar C, Carter D, Stark J, Giles B, Zimmerman R, Ostroff S, Lee B: Seroprevalence following the second wave of pandemic 2009 H1N1 influenza. PLoS Curr Influenza. 2010, RRN1148-10.1371/currents.RRN1148.
11.
Chan YJ, Lee CL, Hwang SJ, Fung CP, Wang FD, Yen DH, Tsai CH, Chen YM, Lee SD: Seroprevalence of antibodies to pandemic (H1N1) 2009 influenza virus among hospital staff in a medical center in Taiwan. J Chin Med Assoc. 2010, 73: 62-66. 10.1016/S1726-4901(10)70003-4.CrossRefPubMed
12.
Allwinn R, Geiler J, Berger A, Cinatl J, Doerr HW: Determination of serum antibodies against swine-origin influenza A virus H1N1/2009 by immunofluorescence, haemagglutination inhibition, and by neutralization tests: how is the prevalence rate of protecting antibodies in humans?. Med Microbiol Immunol. 2010, 199 (2): 117-121. 10.1007/s00430-010-0143-4.CrossRefPubMed
13.
14.
Rowe T, Abernathy RA, Hu-Primmer J, Thompson WW, Lu X, Lim W, Fukuda K, Cox NJ, Katz JM: Detection of antibody to Avian Influenza A (H5N1) virus in human serum by using a combination of serologic assays. J Clin Microbiol. 1999, 37: 937-943.PubMedPubMedCentral
15.
16.
17.
Chen MIC, Lee VJM, Lim W-Y, Barr IG, Lin RTP, Koh GCH, Yap J, Cui L, Cook AR, Laurie K, Tan LWL, Tan BH, Loh J, Shaw R, Durrent C, Chow VTK, Kelso A, Chia KS, Leo YS: 2009 influenza A(H1N1) seroconversion rates and risk factors among distinct adult cohorts in Singapore. JAMA. 2010, 303 (14): 1383-1391. 10.1001/jama.2010.404.CrossRefPubMed
18.
World Health Organization: Seroepidemiological studies of pandemic influenza A (H1N1) 2009 virus. Wkly Epidemiol Rec. 2010, 85: 229-236.
19.
Epstein SL: Prior H1N1 influenza infection and susceptibility of Cleveland family study participants during the H2N2 pandemic of 1957: an experiment of nature. J Infect Dis. 2006, 193: 49-53. 10.1086/498980.CrossRefPubMed
20.
Tang JW, Tambyah PA, Wilder-Smith A, Puong K-Y, Shaw R, Barr IG, Chan K-P: Cross-reactive antibodies to pandemic (H1N1) 2009 virus, Singapore. Emerg Infect Dis. 2010, 16 (5): 874-876.CrossRefPubMedPubMedCentral
21.
Gurav YK, Pawar SD, Chadha MS, Potdar VA, Deshpande AS, Koratkar SS, Hosmani AH, Mishra AC: Pandemic influenza A (H1N1) 2009 outbreak in a residential school in Panchgani, Maharashtra, India. Indian J Med Res. 2010, 132: 67-71.PubMed
22.
Calatayud L, Kurkela S, Neave PE, Brock A, Perkins S, Zuckerman M, Sudhanva M, Bermingham A, Ellis J, Pebody R, Catchpole M, Heathcock R, Maguire H: Pandemic (H1N1) 2009 virus outbreak in a school in London, April-May 2009: an observational study. Epidemiol Infect. 2010, 138: 183-191. 10.1017/S0950268809991191.CrossRefPubMed
23.
Lessler J, Reich NG, Cummings DA, New York City Department of Health and Mental Hygiene Swine Influenza Investigation Team, Nair HP, Jordan HT, Thompson N: Outbreak of 2009 pandemic influenza A (H1N1) at a New York City school. N Engl J Med. 2009, 361: 2628-2636. 10.1056/NEJMoa0906089.CrossRefPubMed
24.
Ghani AC, Baguelin M, Griffin J, Flasche S, Pebody R, van Hoek AJ, Cauchemez S, Hall IM, Donnelly C, Robertson C, White MT, Barrass I, Fraser C, Bermingham A, Truscott J, Ellis J, Jenkins H, Kafatos G, Garske T, Harris R, McMenamin J, Hawkins C, Phin N, Charlett A, Zambon M, Edmonds WJ, Catchpole M, Leach S, White P, Ferguson NM, Cooper B: The early transmission dynamics of H1N1 pdm influenza in the United Kingdom. PLoS Curr Influenza. 2009, RRN1130-
25.
Jiang T, Li X, Liu W, Yu M, Liu J, Yu X, Qin E, Cao W, Leng Q, Qin C: Serum antibody response to the novel influenza A (H1N1) virus in the elderly. Clin Infect Dis. 2010, 50: 285-286. 10.1086/649552.CrossRefPubMed
26.
Chowell G, Bertozzi SM, Colchero MA, Lopez-Gatell H, Alpuche-Aranda C, Hernandez M, Miller MA: Severe respiratory disease concurrent with the circulation of H1N1 influenza. N Engl J Med. 2009, 361: 674-679. 10.1056/NEJMoa0904023.CrossRefPubMed
27.
Cauchemez S, Donnelly CA, Reed C, Ghani AC, Fraser C, Kent CK, Finelli L, Ferguson NM: Household transmission of 2009 pandemic influenza A (H1N1) virus in the United States. N Engl J Med. 2009, 361: 2619-2627. 10.1056/NEJMoa0905498.CrossRefPubMed
28.
Lee VJ, Yap J, Tay JK, Barr I, Gao Q, Ho HJ, Tan BH, Kelly PM, Tambyah PA, Kelso A, Chen MI: Seroconversion and asymptomatic infections during oseltamivir prophylaxis against influenza A H1N1 2009. BMC Infect Dis. 2010,
29.
Flahault A, de Lamballerie X, Hanslik T, Salez N: Symptomatic infections less frequent with H1N1 pdm than with seasonal strains. PLoS Curr Influenza. 2009, RRN1140-10.1371/currents.RRN1140.
30.
Baker MG, Wilson N, Huang QS, Paine S, Lopez L, Bandaranayake D, Tobias M, Mason K, Mackereth GF, Jacobs M, Thornley C, Roberts S, McArthur C: Pandemic influenza A (H1N1)v in New Zealand: the experience from April to August 2009. Euro Surveill. 2009, 14: pii:19319-
31.
Presanis AM, De Angelis D, New York City Swine Flu Investigation Team, Hagy A, Reed C, Riley S, Cooper BS, Finelli L, Biedrzycki P, Lipsitch M: The severity of pandemic H1N1 influenza in the United states, from April to July 2009: a Bayesian analysis. PLoS Med. 2009, 6: e1000207-10.1371/journal.pmed.1000207.CrossRefPubMedPubMedCentral
32.
Clarke SK, Heath RB, Sutton RN, Stuart-Harris CH: Serological studies with Asian strain of influenza A. Lancet. 1958, 1: 814-818. 10.1016/S0140-6736(58)91739-2.CrossRefPubMed
33.
Viboud C, Grais RF, Lafont BA, Miller MA, Simonsen L, Multinational Influenza Seasonal Mortality Study Group: Multinational impact of the 1968 Hong Kong influenza pandemic: evidence for a smoldering pandemic. J Infect Dis. 2005, 192: 233-248. 10.1086/431150.CrossRefPubMed
34.
Fraser C, Donnelly CA, Cauchemez S, Hanage WP, Van Kerkhove MD, Hollingsworth TD, Griffin J, Baggaley RF, Jenkins HE, Lyons EJ, Jombart T, Hinsley WR, Grassly NC, Balloux F, Ghani AC, Ferguson NM, Rambaut A, Pybus OG, Lopez-Gatell H, Alpuche-Aranda CM, Chapela IB, Zavala EP, Guevara DM, Checchi F, Garcia E, Hugonnet S, Roth C, WHO Rapid Pandemic Assessment Collaboration: Pandemic potential of a strain of influenza A (H1N1): early findings. Science. 2009, 324: 1557-1561. 10.1126/science.1176062.CrossRefPubMedPubMedCentral
35.
Yang J, Yang F, Huang F, Wang J, Jin Q: Subclinical infection with the novel influenza A (H1N1) virus. Clin Infect Dis. 2009, 49: 1622-1623. 10.1086/644775.CrossRefPubMed
Metadata
Title
Seroepidemiology of pandemic influenza A (H1N1) 2009 virus infections in Pune, India
Authors
Babasaheb V Tandale
Shailesh D Pawar
Yogesh K Gurav
Mandeep S Chadha
Santosh S Koratkar
Vijay N Shelke
Akhilesh C Mishra
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2010
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-10-255

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