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Intensive Care Medicine
Published in: Intensive Care Medicine 8/2010

01-08-2010 | Correspondence

Septic shock due to Legionella pneumophila serogroup 2: usefulness of molecular biology for diagnosis, treatment and epidemiological investigation

Authors: Florence Grattard, Séverine Allegra, Jerome Morel, Isabelle Court-Fortune, Christian Auboyer, Bruno Pozzetto, Philippe Berthelot

Published in: Intensive Care Medicine | Issue 8/2010

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Excerpt

During the last 10 years, nucleic acid detection methods have been shown to offer a good alternative to the diagnosis of legionellosis (reviewed by Diederen in [1]), especially when non-L. pneumophila sg1 strains are involved [2]. The case presented herein is illustrative of the benefit of using molecular techniques to diagnose and manage such infections. …
Literature
1.
2.
Miller LA, Beebe JL, Butler JC, Martin W, Benson R, Hoffman RE, Fields BS (1993) Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever. J Infect Dis 168:769–772PubMed
3.
Jonas D, Rosenbaum A, Weyrich S, Bhakdi S (1995) Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid. J Clin Microbiol 33:1247–1252PubMed
4.
Association Française de Normalisation (2003) Water quality-detection and enumeration of Legionella spp. and Legionella pneumophila. Method by direct inoculation and after concentration by membrane filtration or centrifugation. AFNOR NF T90-431. Paris, France
5.
Grattard F, Ginevra C, Riffard S, Ros A, Jarraud S, Etienne J, Pozzetto B (2006) Analysis of the genetic diversity of Legionella by sequencing the 23S–5S ribosomal intergenic spacer region: from phylogeny to direct identification of isolates at the species level from clinical specimens. Microbes Infect 8:73–83CrossRefPubMed
Metadata
Title
Septic shock due to Legionella pneumophila serogroup 2: usefulness of molecular biology for diagnosis, treatment and epidemiological investigation
Authors
Florence Grattard
Séverine Allegra
Jerome Morel
Isabelle Court-Fortune
Christian Auboyer
Bruno Pozzetto
Philippe Berthelot
Publication date
01-08-2010
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 8/2010
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-010-1859-8

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