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Current Osteoporosis Reports
Published in: Current Osteoporosis Reports 2/2018

01-04-2018 | Genetics (M Johnson and S Ralston, Section Editors)

Screening Gene Knockout Mice for Variation in Bone Mass: Analysis by μCT and Histomorphometry

Authors: David W. Rowe, Douglas J. Adams, Seung-Hyun Hong, Caibin Zhang, Dong-Guk Shin, C. Renata Rydzik, Li Chen, Zhihua Wu, Gaven Garland, Dana A. Godfrey, John P. Sundberg, Cheryl Ackert-Bicknell

Published in: Current Osteoporosis Reports | Issue 2/2018

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Abstract

Purpose of review

The international mouse phenotyping consortium (IMPC) is producing defined gene knockout mouse lines. Here, a phenotyping program is presented that is based on micro-computed tomography (μCT) assessment of distal femur and vertebra. Lines with significant variation undergo a computer-based bone histomorphometric analysis.

Recent findings

Of the 220 lines examined to date, approximately 15% have a significant variation (high or low) by μCT, most of which are not identified by the IMPC screen. Significant dimorphism between the sexes and bone compartments adds to the complexity of the skeletal findings. The μCT information that is posted at www.​bonebase.​org can group KOMP lines with similar morphological features. The histological data is presented in a graphic form that associates the cellular features with a specific anatomic group.

Summary

The web portal presents a bone-centric view appropriate for the skeletal biologist/clinician to organize and understand the large number of genes that can influence skeletal health. Cataloging the relative severity of each variant is the first step towards compiling the dataset necessary to appreciate the full polygenic basis of degenerative bone disease.
Appendix
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Metadata
Title
Screening Gene Knockout Mice for Variation in Bone Mass: Analysis by μCT and Histomorphometry
Authors
David W. Rowe
Douglas J. Adams
Seung-Hyun Hong
Caibin Zhang
Dong-Guk Shin
C. Renata Rydzik
Li Chen
Zhihua Wu
Gaven Garland
Dana A. Godfrey
John P. Sundberg
Cheryl Ackert-Bicknell
Publication date
01-04-2018
Publisher
Springer US
Published in
Current Osteoporosis Reports / Issue 2/2018
Print ISSN: 1544-1873
Electronic ISSN: 1544-2241
DOI
https://doi.org/10.1007/s11914-018-0421-4

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