Published in:
01-02-2012 | Original Article
Scavenger receptor expressions in the kidneys of mice with lipoprotein glomerulopathy
Published in: Clinical and Experimental Nephrology | Issue 1/2012
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Background
To clarify whether dysfunction of the scavenger receptor (SR) participates in the development of lipoprotein glomerulopathy (LPG) in immunoglobulin Fc receptor γ chain (FcRγ)-deficient mice [FcRγ knock-out (KO) mice] with induced chronic graft-versus-host disease (cGVHD).
Method
In wild-type (WT) and FcRγ KO C57BL/6 mice, cGVHD was induced by injection of lymphoid cells from donor Bm12 mice. At 6 months after injection, the mice were sacrificed and histologically examined. Total RNA was extracted from the kidneys and cytokine, chemokine, and SR transcript expressions were evaluated by reverse transcription-polymerase chain reaction.
Results
Three of 4 female cGVHD(+)/FcRγ KO mice presented LPG in >60% of glomeruli. cGVHD(−) and cGVHD(+)/WT mice did not show LPG. The SRs CD36, CD68, and CXCL16 showed a significant difference in the values of their transcripts between cGVHD(+)/WT and cGVHD(+)/FcRγ KO mice. Among them, only CD36 showed a drastic decline of mRNA expressions in cGVHD(+)/FcRγ KO mice.
Conclusion
CD36 may play a crucial role in the development of LPG in FcRγ KO mice with cGVHD. In addition to the apolipoprotein E mutation, dysfunction of lipid clearance in the kidney might be one of the factors for the development of LPG.