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European Journal of Applied Physiology

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Open Access 01-02-2018 | Original Article

Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

Authors: Jamie K. Pugh, Steve H. Faulkner, Mark C. Turner, Myra A. Nimmo

Published in: European Journal of Applied Physiology | Issue 2/2018

Abstract

Purpose

Sarcopenia can begin from the 4–5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone.

Methods

Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HR_max on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7⁺ cells) and active (MyoD⁺ cells) satellite cell number using immunofluorescence microscopy.

Results

Type-I-specific Pax7⁺ (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD⁺ (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7⁺ and MyoD⁺ cell number remained unchanged after both exercise trials (all P ≥ 0.13).

Conclusion

Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.

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Title: Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals
Authors: Jamie K. Pugh
Steve H. Faulkner
Mark C. Turner
Myra A. Nimmo
Publication date: 01-02-2018
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 2/2018
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-017-3721-y

At a glance: The STEP trials

Springer Medicine

Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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