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Translational Stroke Research
Published in: Translational Stroke Research 2/2011

01-06-2011

Sampling of CSF via the Cisterna Magna and Blood Collection via the Heart Affects Brain Water Content in a Rat SAH Model

Authors: Kamil Duris, Anatol Manaenko, Hidenori Suzuki, William Rolland, Jiping Tang, John H. Zhang

Published in: Translational Stroke Research | Issue 2/2011

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Abstract

The aim of this study was to evaluate whether sampling of cerebrospinal fluid (CSF) via the cisterna magna and of blood via the heart affects brain water content in a rat subarachnoid hemorrhage (SAH) model. Twenty-nine animals were divided into four groups: sham-operated group with sampling of CSF and blood (Sham S+), sham-operated group without sampling of CSF and blood (Sham S−), SAH group with sampling of CSF and blood (SAH S+), and SAH without sampling of CSF and blood (SAH S−). SAH was induced via endovascular perforation of the left internal carotid artery bifurcation. Cerebrospinal fluid via the cisterna magna and blood via cardiac puncture was collected in the Sham S+ and SAH S+ groups before killing the animals for brain water content measurements. Left hemisphere brain water content was significantly higher in the SAH S− group compared with the Sham S− group (p < 0.05) and in Sham S+ group compared with the Sham S− group (p < 0.05). There was no significant difference in brain water content of the left hemisphere between the SAH S+ and Sham S+ groups (p = NS). There was no significant difference in brain water content in other parts of brains. Sampling of CSF and blood affected brain water content in Sham animals and therefore it is not accurate to use these values from Sham animals for comparison with SAH animals.
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Metadata
Title
Sampling of CSF via the Cisterna Magna and Blood Collection via the Heart Affects Brain Water Content in a Rat SAH Model
Authors
Kamil Duris
Anatol Manaenko
Hidenori Suzuki
William Rolland
Jiping Tang
John H. Zhang
Publication date
01-06-2011
Publisher
Springer-Verlag
Published in
Translational Stroke Research / Issue 2/2011
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI
https://doi.org/10.1007/s12975-010-0063-z

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