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BMC Oral Health

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Open Access 01-12-2021 | Root Canal Sealer | Research article

An in vitro study on the effects of serum proteins on Enterococcus faecalis adhesion to three types of root sealers and gutta-percha

Authors: Xinwei Lin, Danlu Chi, Qimei Gong, Zhongchun Tong

Published in: BMC Oral Health | Issue 1/2021

Abstract

Background

The extrusion of overfilled materials that extend beyond the apical foramina into the periradicular tissue may serve as a reservoir for bacterial adhesion and further affect recovery from periapical diseases. The aim of this study was to evaluate the effects of serum proteins on Enterococcus faecalis adhesion and survival on the surface of a calcium hydroxide-based root canal sealer (Apexit Plus), an epoxy resin sealer (AH-Plus) and a bioceramic sealer (iRoot SP).

Methods

Apexit Plus, AH-Plus and iRoot SP were evenly coated on gutta-percha, using gutta-percha alone as the control. After root canal sealer setting, the number of E. faecalis adhering to the root canal sealers and gutta-percha was counted in fetal bovine serum (FBS) or tryptic soy broth supplemented with 1% glucose (TSBG) by viable cell plate counts. The morphology of 7-day-old E. faecalis biofilms in FSB and TSBG was observed by scanning electron microscopy (SEM). Furthermore, E. faecalis biofilms on the three root canal sealers were labeled with a LIVE/DEAD BacLight™ Bacterial Viability Kit, and the ratios of viable to dead cells were analyzed using laser scanning microscopy operative software (Zen software).

Results

In the assays, after 1 and 7 days, the number of E. faecalis adhering to the root canal sealers or gutta-percha in FBS were significantly lower than those in TSBG (P < 0.05). In FBS, E. faecalis adhesion to iRoot SP and gutta-percha was reduced to a greater extent than that adhered to Apexit Plus and AH-Plus. Few E. faecalis accumulated on iRoot SP in FBS, whereas many bacteria assembled on iRoot SP and formed biofilms in TSBG. The ratio of viable cells in the E. faecalis biofilm on iRoot SP was the lowest.

Conclusions

Calcium hydroxide-based root canal sealers, epoxy resin sealers and bioceramic sealers may provide a substrate for E. faecalis adhesion, and the bioceramic sealer in this study showed the least E. faecalis adhesion in the presence of serum proteins compared to the other two sealers.

Donnermeyer D, Vahdat-Pajouh N, Schafer E, et al. Influence of the final irrigation solution on the push-out bond strength of calcium silicate-based, epoxy resin-based and silicone-based endodontic sealers. Odontology. 2019;107(2):231–6.PubMedCrossRef

Nosrat A, Verma P, Hicks ML, et al. Variations of palatal canal morphology in maxillary molars: a case series and literature review. J Endod. 2017;43(11):1888–96.PubMedCrossRef

Komabayashi T, Colmenar D, Cvach N, et al. Comprehensive review of current endodontic sealers. Dent Mater J. 2020;39(5):703–20.PubMedCrossRef

Salz U, Poppe D, Sbicego S, et al. Sealing properties of a new root canal sealer. Int Endod J. 2009;42(12):1084–9.PubMedCrossRef

Senges C, Wrbas KT, Altenburger M, et al. Bacterial and Candida albicans adhesion on different root canal filling materials and sealers. J Endod. 2011;37(9):1247–52.PubMedCrossRef

Alsubait S, Albader S, Alajlan N, et al. Comparison of the antibacterial activity of calcium silicate- and epoxy resin-based endodontic sealers against Enterococcus faecalis biofilms: a confocal laser-scanning microscopy analysis. Odontology. 2019;107(4):513–20.PubMedCrossRef

Huang Y, Li X, Mandal P, et al. The in vitro antimicrobial activities of four endodontic sealers. BMC Oral Health. 2019;19(1):118.PubMedPubMedCentralCrossRef

Lee JK, Kwak SW, Ha JH, et al. Physicochemical properties of epoxy resin-based and bioceramic-based root canal sealers. Bioinorg Chem Appl. 2017;2017:2582849.PubMedPubMedCentralCrossRef

Schafer E, Zandbiglari T. Solubility of root-canal sealers in water and artificial saliva. Int Endod J. 2003;36(10):660–9.PubMedCrossRef

10.

Donnelly A, Sword J, Nishitani Y, et al. Water sorption and solubility of methacrylate resin-based root canal sealers. J Endod. 2007;33(8):990–4.PubMedCrossRef

11.

Kapralos V, Koutroulis A, Orstavik D, et al. Antibacterial Activity of endodontic sealers against planktonic bacteria and bacteria in biofilms. J Endod. 2018;44(1):149–54.PubMedCrossRef

12.

Gallusi G, Campanella V, Montemurro E, et al. Antibacterial activity of first and latest generation bioceramic sealers on the elimination of enterococcus faecalis: an in vitro study. J Biol Regul Homeost Agents. 2020;34(3 Suppl. 1):73–9.PubMed

13.

Bukhari S, Karabucak B. The antimicrobial effect of bioceramic sealer on an 8-week matured Enterococcus faecalis biofilm attached to root canal dentinal surface. J Endod. 2019;45(8):1047–52.PubMedCrossRef

14.

Molven O, Halse A, Fristad I, et al. Periapical changes following root-canal treatment observed 20–27 years postoperatively. Int Endod J. 2002;35(9):784–90.PubMedCrossRef

15.

Nair PN, Sjogren U, Krey G, et al. Therapy-resistant foreign body giant cell granuloma at the periapex of a root-filled human tooth. J Endod. 1990;16(12):589–95.PubMedCrossRef

16.

Sjogren U, Sundqvist G, Nair PN. Tissue reaction to gutta-percha particles of various sizes when implanted subcutaneously in guinea pigs. Eur J Oral Sci. 1995;103(5):313–21.PubMedCrossRef

17.

Ricucci D, Rocas IN, Alves FR, et al. Apically extruded sealers: fate and influence on treatment outcome. J Endod. 2016;42(2):243–9.PubMedCrossRef

18.

Fristad I, Molven O, Halse A. Nonsurgically retreated root filled teeth–radiographic findings after 20–27 years. Int Endod J. 2004;37(1):12–8.PubMedCrossRef

19.

Bjorndal L, Amaloo C, Markvart M, et al. maxillary sinus impaction of a core carrier causing sustained apical periodontitis, sinusitis, and nasal stenosis: a 3-year follow-up. J Endod. 2016;42(12):1851–8.PubMedCrossRef

20.

Guerreiro-Tanomaru JM, de Faria-Junior NB, Duarte MA, et al. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. J Endod. 2013;39(3):346–50.PubMedCrossRef

21.

Noiri Y, Ehara A, Kawahara T, et al. Participation of bacterial biofilms in refractory and chronic periapical periodontitis. J Endod. 2002;28(10):679–83.PubMedCrossRef

22.

Schaeffer MA, White RR, Walton RE. Determining the optimal obturation length: a meta-analysis of literature. J Endod. 2005;31(4):271–4.PubMedCrossRef

23.

Candeiro GTM, Moura-Netto C, D’Almeida-Couto RS, et al. Cytotoxicity, genotoxicity and antibacterial effectiveness of a bioceramic endodontic sealer. Int Endod J. 2016;49(9):858–64.PubMedCrossRef

24.

Yoshino P, Nishiyama CK, Modena KC, et al. In vitro cytotoxicity of white MTA, MTA Fillapex(R) and Portland cement on human periodontal ligament fibroblasts. Braz Dent J. 2013;24(2):111–6.PubMedCrossRef

25.

Xu J, He J, Shen Y, et al. Influence of endodontic procedure on the adherence of Enterococcus faecalis. J Endod. 2019;45(7):943–9.PubMedCrossRef

26.

Beyth N, Kesler-Shvero D, Zaltsman N, et al. Rapid kill-novel endodontic sealer and Enterococcus faecalis. PLoS ONE. 2013;8(11):785.CrossRef

27.

Bodrumlu E, Semiz M. Antibacterial activity of a new endodontic sealer against Enterococcus faecalis. J Can Dent Assoc. 2006;72(7):637.PubMed

28.

Hoelscher AA, Bahcall JK, Maki JS. In vitro evaluation of the antimicrobial effects of a root canal sealer-antibiotic combination against Enterococcus faecalis. J Endod. 2006;32(2):145–7.PubMedCrossRef

29.

Crabbe A, Liu Y, Matthijs N, et al. Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. Sci Rep. 2017;7:43321.PubMedPubMedCentralCrossRef

30.

Pappen FG, Qian W, Aleksejuniene J, et al. Inhibition of sodium hypochlorite antimicrobial activity in the presence of bovine serum albumin. J Endod. 2010;36(2):268–71.PubMedCrossRef

31.

Tong Z, Zhang Y, Wei X. The effect of human serum and dentin powder alone or in combination on the antibacterial activity of sodium hypochlorite against Enterococcus faecalis. Arch Oral Biol. 2019;97:72–6.PubMedCrossRef

32.

Torres FFE, Guerreiro-Tanomaru JM, Bosso-Martelo R, et al. Solubility, Porosity, Dimensional and Volumetric Change of Endodontic Sealers. Braz Dent J. 2019;30(4):368–73.PubMedCrossRef

33.

McMichen FR, Pearson G, Rahbaran S, et al. A comparative study of selected physical properties of five root-canal sealers. Int Endod J. 2003;36(9):629–35.PubMedCrossRef

34.

AlShwaimi E, Bogari D, Ajaj R, et al. In vitro antimicrobial effectiveness of root canal sealers against Enterococcus faecalis: a systematic review. J Endod. 2016;42(11):1588–97.PubMedCrossRef

35.

Chogle S, Mickel AK, Huffaker SK, et al. An in vitro assessment of iodoform gutta-percha. J Endod. 2005;31(11):814–6.PubMedCrossRef

36.

Lohbauer U, Gambarini G, Ebert J, et al. Calcium release and pH-characteristics of calcium hydroxide plus points. Int Endod J. 2005;38(10):683–9.PubMedCrossRef

37.

Lui JN, Sae-Lim V, Song KP, et al. In vitro antimicrobial effect of chlorhexidine-impregnated gutta percha points on Enterococcus faecalis. Int Endod J. 2004;37(2):105–13.PubMedCrossRef

38.

Melker KB, Vertucci FJ, Rojas MF, et al. Antimicrobial efficacy of medicated root canal filling materials. J Endod. 2006;32(2):148–51.PubMedCrossRef

39.

George S, Basrani B, Kishen A. Possibilities of gutta-percha-centered infection in endodontically treated teeth: an in vitro study. J Endod. 2010;36(7):1241–4.PubMedCrossRef

40.

Takemura N, Noiri Y, Ehara A, et al. Single species biofilm-forming ability of root canal isolates on gutta-percha points. Eur J Oral Sci. 2004;112(6):523–9.PubMedCrossRef

41.

Ravi Chandra PV, Kumar VH, Reddy SJ, et al. Biofilm forming capacity of Enterococcus faecalis on Gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study. Dent Res J (Isfahan). 2015;12(4):331–6.CrossRef

42.

Braga JM, Oliveira RR, de Castro MR, et al. Assessment of the cytotoxicity of a mineral trioxide aggregate-based sealer with respect to macrophage activity. Dent Traumatol. 2015;31(5):390–5.PubMedCrossRef

43.

Bernath M, Szabo J. Tissue reaction initiated by different sealers. Int Endod J. 2003;36(4):256–61.PubMedCrossRef

Title: An in vitro study on the effects of serum proteins on Enterococcus faecalis adhesion to three types of root sealers and gutta-percha
Authors: Xinwei Lin
Danlu Chi
Qimei Gong
Zhongchun Tong
Publication date: 01-12-2021
Publisher: BioMed Central
Keyword: Root Canal Sealer
Published in: BMC Oral Health / Issue 1/2021
Electronic ISSN: 1472-6831
DOI: https://doi.org/10.1186/s12903-021-01992-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

An in vitro study on the effects of serum proteins on Enterococcus faecalis adhesion to three types of root sealers and gutta-percha

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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