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Open Access 01-05-2024 | Root Canal Debridement | Research

Elimination of E. faecalis with NaOCl versus chlorhexidine gluconate from primary molar root canal systems: an ex vivo model study

Authors: Shmueli Aviv, Yaya Alin, Lam Neta, Haim Yael, Zamsky Lada, Fux Noy Avia, Ram Diana, Moskovitz Moti, Polak David

Published in: Clinical Oral Investigations | Issue 5/2024

Abstract

Objectives

This ex vivo human study aimed to evaluate the efficacy of NaOCl and chlorhexidine gluconate (CHG) irrigations in eliminating Enterococcus faecalis from the RCS of primary molars.

Materials and methods

Disinfected extracted primary molars were inoculated with E. faecalis for 24 h. Then, the RCS samples were then irrigated with either 2.5% NaOCl, 0.2% and 2% CHG, or sham saline. The samples were collected immediately after irrigation; and 24 h later, the bacterial viability and counts were measured using blood agar and qRT-PCR, respectively. Histological sections were used to measure E. faecalis penetration and viability in dentin tubules using fluorescence microscopy.

Results

The recovery of viable E. faecalis after the irrigation of the primary molars showed more significant bactericidal effects of NaOCl and 0.2% and 2% CHG than of saline. Immediately after the irrigation, the NaOCl group showed the greatest reduction in E. faecalis; and 24 h later, all the groups had lower viable E. faecalis than the saline control. The bacterial penetration was also lowest in the NaOCl group, although there was no difference in bacterial viability in the tubules between the groups.

Conclusion

In primary teeth, NaOCl and CHG showed similar degrees of bacterial elimination efficacy in terms of E.faecalis.

Clinical relevance

Within the limitations of this study, NaOCl and CHG have the similar ability to perform endodontic irrigation of primary ex vivo teeth regarding the elimination of E.faecalis, but NaOCl penetrates dentin tubules better.

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Title: Elimination of E. faecalis with NaOCl versus chlorhexidine gluconate from primary molar root canal systems: an ex vivo model study
Authors: Shmueli Aviv
Yaya Alin
Lam Neta
Haim Yael
Zamsky Lada
Fux Noy Avia
Ram Diana
Moskovitz Moti
Polak David
Publication date: 01-05-2024
Publisher: Springer Berlin Heidelberg
Keywords: Root Canal Debridement
Root Canal Debridement
Endodontic Irrigation
Published in: Clinical Oral Investigations / Issue 5/2024
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-024-05621-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Elimination of E. faecalis with NaOCl versus chlorhexidine gluconate from primary molar root canal systems: an ex vivo model study

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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