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Radiation Oncology
Published in: Radiation Oncology 1/2009

Open Access 01-12-2009 | Research

Role of p53 mutation in the effect of boron neutron capture therapy on oral squamous cell carcinoma

Authors: Yusei Fujita, Itsuro Kato, Soichi Iwai, Koji Ono, Minoru Suzuki, Yoshinori Sakurai, Ken Ohnishi, Takeo Ohnishi, Yoshiaki Yura

Published in: Radiation Oncology | Issue 1/2009

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Abstract

Background

Boron neutron capture therapy (BNCT) is a selective radiotherapy, being effective for the treatment of even advanced malignancies in head and neck regions as well as brain tumors and skin melanomas. To clarify the role of p53 gene, the effect of BNCT on oral squamous cell carcinoma (SCC) cells showing either wild- (SAS/neo) or mutant-type (SAS/mp53) p53 was examined.

Methods

Cells were exposed to neutron beams in the presence of boronophenylalanine (BPA) at Kyoto University Research Reactor. Treated cells were monitored for modulations in colony formation, proliferation, cell cycle, and expression of cell cycle-associated proteins.

Results

When SAS/neo and SAS/mp53 cells were subjected to BNCT, more suppressive effects on colony formation and cell viability were observed in SAS/neo compared with SAS/mp53 cells. Cell cycle arrest at the G1 checkpoint was observed in SAS/neo, but not in SAS/mp53. Apoptotic cells increased from 6 h after BNCT in SAS/neo and 48 h in SAS/mp53 cells. The expression of p21 was induced in SAS/neo only, but G2 arrest-associated proteins including Wee1, cdc2, and cyclin B1 were altered in both cell lines.

Conclusion

These results indicate that oral SCC cells with mutant-type are more resistant to BNCT than those with wild-type p53, and that the lack of G1 arrest and related apoptosis may contribute to the resistance. At a physical dose affecting the cell cycle, BNCT inhibits oral SCC cells in p53-dependent and -independent manners.
Appendix
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Metadata
Title
Role of p53 mutation in the effect of boron neutron capture therapy on oral squamous cell carcinoma
Authors
Yusei Fujita
Itsuro Kato
Soichi Iwai
Koji Ono
Minoru Suzuki
Yoshinori Sakurai
Ken Ohnishi
Takeo Ohnishi
Yoshiaki Yura
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Radiation Oncology / Issue 1/2009
Electronic ISSN: 1748-717X
DOI
https://doi.org/10.1186/1748-717X-4-63

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