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01-02-2016 | Original Article

Role of immune tolerance in BALB/c mice with anaphylactic shock after Echinococcus granulosus infection

Authors: Qin Zhang, Jian-Rong Ye, Hai-Mei Ma, Jian-Jiang Wu, Tao Jiang, Hong Zheng

Published in: Immunologic Research | Issue 1/2016

Abstract

This study tested the hypothesis that immune tolerance mediated by regulatory T (Treg) cells is protective against cystic echinococcosis (CE)-induced anaphylactic shock. BALB/c mice were inoculated with protoscoleces of Echinococcus granulosus. After 3 months, the presence of cysts in the peritoneal cavity was confirmed after which a subset of mice was sensitized using a cyst fluid suspension to induce anaphylactic shock. While IgE levels were significantly higher in both groups inoculated with E. granulosus as compared to the healthy control group (both P < 0.01), sensitized mice had higher IgE levels as compared with those with E. granulosus alone (P < 0.05). Mice inoculated with E. granulosus alone and sensitized mice both had significantly higher histamine levels as compared to the healthy controls. The proportion of CD4⁺CD25⁺Foxp3⁺ Treg cells relative to CD4⁺ cells was significantly higher in mice inoculated with E. granulosus alone (P < 0.0167); significantly higher interleukin-10 (IL-10) and tumor growth factor-β (TGF-β1) levels were also noted in this group (all P < 0.01). In contrast, IL-13 and IL-17A levels were significantly higher in the sensitized mice (both P < 0.05). Taken together, these data suggest that the biphasic changes in Treg cell and cytokine levels may be associated with anaphylactic shock induced by CE.

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Title: Role of immune tolerance in BALB/c mice with anaphylactic shock after Echinococcus granulosus infection
Authors: Qin Zhang
Jian-Rong Ye
Hai-Mei Ma
Jian-Jiang Wu
Tao Jiang
Hong Zheng
Publication date: 01-02-2016
Publisher: Springer US
Published in: Immunologic Research / Issue 1/2016
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-015-8741-2

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Role of immune tolerance in BALB/c mice with anaphylactic shock after Echinococcus granulosus infection

Abstract

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Springer Medicine

Abstract

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