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Journal of Assisted Reproduction and Genetics

Published in:

01-02-2014 | Gamete Biology

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Authors: Ayan Mukherjee, Hrudananda Malik, Ambika P. Saha, Amit Dubey, Dinesh Kumar Singhal, Samuel Boateng, Shrabani Saugandhika, Sudarshan Kumar, Sachinandan De, Shyamal Kanti Guha, Dhruba Malakar

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2014

Abstract

Purpose

The aim of the present study was to determine whether supplementation of resveratrol, a stilbenoid antioxidant with therapeutic significance, influences goat (Capra hircus) oocyte maturation and subsequent embryonic development and expression of apoptosis and early embryonic development-related genes.

Methods

Five different concentrations of resveratrol (0.1, 0.25, 0.5, 2.0 and 5.0 μM) were used in in vitro maturation (IVM) medium. Cell tracker blue and 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA) fluorescent stains were used to assay intracellular glutathione and reactive oxygen species levels in mature oocytes. Parthenogenetic activation and hand-made cloning were performed to check the developmental potential following resveratrol treatment. We used quantitative real-time PCR to analyze embryonic gene expression.

Result

Compared to control, no significant improvement was observed in nuclear maturation in resveratrol-treated groups and at 5.0 μM concentration maturation rate decreased significantly (P < 0.05). But resveratrol treatment at the concentrations of 0.25, 0.5 μM significantly reduced intracellular ROS, and increased GSH concentrations. Oocytes treated with 0.25, 0.5 μM resveratrol when subsequently used for PA and HMC, higher extent of blastocyst yields were observed. Expression analysis of proapoptotic (Bax) gene in mature oocytes, cumulus cells, and HMC-derived blastocysts revealed lesser transcript abundances in various resveratrol-treated groups., however no change in the same was observed for antiapoptotic gene (Bcl2). Differential expression of genes associated with developmental competence and nuclear reprogramming was also observed in HMC-derived blastocysts.

Conclusion

Our results show that resveratrol treatment at optimum concentrations (0.25 and 0.5 μM) during IVM produced beneficial microenvironment within oocytes by increasing the intracellular GSH, decreasing ROS level and this in turn, stimulated embryonic development and regulated gene expression.

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Title: Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression
Authors: Ayan Mukherjee
Hrudananda Malik
Ambika P. Saha
Amit Dubey
Dinesh Kumar Singhal
Samuel Boateng
Shrabani Saugandhika
Sudarshan Kumar
Sachinandan De
Shyamal Kanti Guha
Dhruba Malakar
Publication date: 01-02-2014
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2014
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0116-9

At a glance: The STEP trials

Springer Medicine

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Abstract

Purpose

Methods

Result

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Result

Conclusion

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