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Open Access 01-12-2012 | Original research

Reproducibility of quantitative (R)-[¹¹C]verapamil studies

Authors: Daniëlle ME van Assema, Mark Lubberink, Ronald Boellaard, Robert C Schuit, Albert D Windhorst, Philip Scheltens, Bart NM van Berckel, Adriaan A Lammertsma

Published in: EJNMMI Research | Issue 1/2012

Abstract

Background

P-glycoprotein [Pgp] dysfunction may be involved in neurodegenerative diseases, such as Alzheimer's disease, and in drug resistant epilepsy. Positron emission tomography using the Pgp substrate tracer (R)-[¹¹C]verapamil enables in vivo quantification of Pgp function at the human blood-brain barrier. Knowledge of test-retest variability is important for assessing changes over time or after treatment with disease-modifying drugs. The purpose of this study was to assess reproducibility of several tracer kinetic models used for analysis of (R)-[¹¹C]verapamil data.

Methods

Dynamic (R)-[¹¹C]verapamil scans with arterial sampling were performed twice on the same day in 13 healthy controls. Data were reconstructed using both filtered back projection [FBP] and partial volume corrected ordered subset expectation maximization [PVC OSEM]. All data were analysed using single-tissue and two-tissue compartment models. Global and regional test-retest variability was determined for various outcome measures.

Results

Analysis using the Akaike information criterion showed that a constrained two-tissue compartment model provided the best fits to the data. Global test-retest variability of the volume of distribution was comparable for single-tissue (6%) and constrained two-tissue (9%) compartment models. Using a single-tissue compartment model covering the first 10 min of data yielded acceptable global test-retest variability (9%) for the outcome measure K ₁. Test-retest variability of binding potential derived from the constrained two-tissue compartment model was less robust, but still acceptable (22%). Test-retest variability was comparable for PVC OSEM and FBP reconstructed data.

Conclusion

The model of choice for analysing (R)-[¹¹C]verapamil data is a constrained two-tissue compartment model.

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Title: Reproducibility of quantitative (R)-[11C]verapamil studies
Authors: Daniëlle ME van Assema
Mark Lubberink
Ronald Boellaard
Robert C Schuit
Albert D Windhorst
Philip Scheltens
Bart NM van Berckel
Adriaan A Lammertsma
Publication date: 01-12-2012
Publisher: Springer Berlin Heidelberg
Published in: EJNMMI Research / Issue 1/2012
Electronic ISSN: 2191-219X
DOI: https://doi.org/10.1186/2191-219X-2-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Reproducibility of quantitative (R)-[¹¹C]verapamil studies

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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