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Urolithiasis
Published in: Urolithiasis 5/2016

01-10-2016 | Original Paper

Renal tubular injury induced by ischemia promotes the formation of calcium oxalate crystals in rats with hyperoxaluria

Authors: Yanwei Cao, Wanpeng Liu, Limei Hui, Jianjun Zhao, Xuecheng Yang, Yonghua Wang, Haitao Niu

Published in: Urolithiasis | Issue 5/2016

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Abstract

Hyperoxaluria and cell injury are key factors in urolithiasis. Oxalate metabolism may be altered by renal dysfunction and therefore, impact the deposition of calcium oxalate (CaOx) crystals. We investigated the relationship of renal function, oxalate metabolism and CaOx crystal deposition in renal ischemia. One hundred male Sprague–Dawley rats were randomly divided into four groups. Hyperoxaluria model (Group A and B) was established by feeding rats with 0.75 % ethylene glycol (EG). The left renal pedicle was clamped for 30 min to establish renal ischemia Groups (B and C), while Groups A and D underwent sham operation. Then, serum and urine oxalate (Ox), creatinine (Cr) and urea nitrogen (UN) levels were evaluated by liquid chromatography mass spectrometry (LCMS) and ion mass spectrum (IMS) at days 0, 2, 4, 7, and 14. CaOx crystallization was assessed by transmission electron microscope (TEM). A temporal and significant increase of serum Cr and UN levels was observed in Groups B and C compared to values obtained for Groups A and D (P < 0.05). Ox levels in serum and urine were significantly higher in Groups A and B than in the other two groups from day 7 (P < 0.05). In addition, CaOx crystallization was observed in both Groups A and B, but Group B showed earlier and more pronounced crystal deposition in the renal tissue. Our results indicated that renal tubular injury induced by renal ischemia might not affect Ox levels but could promote CaOx crystal retention under hyperoxaluria.
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Metadata
Title
Renal tubular injury induced by ischemia promotes the formation of calcium oxalate crystals in rats with hyperoxaluria
Authors
Yanwei Cao
Wanpeng Liu
Limei Hui
Jianjun Zhao
Xuecheng Yang
Yonghua Wang
Haitao Niu
Publication date
01-10-2016
Publisher
Springer Berlin Heidelberg
Published in
Urolithiasis / Issue 5/2016
Print ISSN: 2194-7228
Electronic ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-016-0876-7

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