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Published in: Lung 5/2006

01-10-2006 | PULMONARY VASCULAR ENDOTHELIUM

Release of Soluble E-Selectin from Activated Endothelial Cells upon Apoptosis

Authors: Elizabeth O. Harrington, Tihomir Stefanec, Julie Newton, Sharon Rounds

Published in: Lung | Issue 5/2006

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Abstract

Circulating soluble E-selectin is increased in diseases associated with endothelial apoptosis such as sepsis and acute respiratory distress syndrome. We investigated the mechanism by which endothelial cell (EC) apoptosis may promote soluble E-selectin release. We found that serum deprivation of EC caused apoptosis, yet it did not induce E-selectin EC surface expression. Tumor necrosis factor-α (TNFα) significantly increased EC E-selectin surface expression. Soluble E-selectin was noted, however, only in the medium of TNFα-activated, apoptotic EC. Preincubation of the EC with the caspase inhibitor z-VAD-fmk significantly attenuated soluble E-selectin levels in the culture medium of TNFα-activated, apoptotic EC, but it had no effect on E-selectin surface expression. These results indicate that TNFα activation, but not apoptosis, is necessary for E-selectin surface expression in EC. Furthermore, E-selectin release from EC requires caspase-3 activation. Thus, increased concentrations of circulating E-selectin in serum may serve as a marker for endothelial apoptosis in certain disease states.
Literature
1.
Bellas R, Harrington E, Sheahan K, et al. (2002) FAK blunts adenosine-homocysteine-induced endothelial cell apoptosis: requirement for PI 3-kinase. Am J Physiol 282: LI135–LI142
2.
Benekli M, Gullu I, Tekuzman G, et al. (1998) Circulating intercellular adhesion molecule-1 and E-selectin levels in gastric cancer. Br J Cancer 78:267–271PubMedCrossRef
3.
Bevilacqua M, Stengelin S, Gimbrone MJ, Seed B (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1160–1165PubMedCrossRef
4.
Bloom B, Nelson S, Eisenberg D, Alario A (2005) Soluble intercellular adhesion molecule-1 and E-selectin as markers of disease activity and endothelial activation in juvenile idiopathic arthritis. J Rheumatol 32:366–372PubMed
5.
Boldt J, Muller M, Kuhn D, Linke L, Hempelmann G (1996) Circulating adhesion molecules in the critically ill: a comparison between trauma and sepsis patients. Intensive Care Med 22:122–128PubMedCrossRef
6.
Cines D, Pollak E, Buck C, et al. (1998) Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 91:3527–3561PubMed
7.
Garton K, Gough P, Philalay J, et al. (2003) Stimulated shedding of vascular cell adhesion molecule l (VCAM-1) is mediated by tumor necrosis factor-α-converting enzyme (ADAM 17). J Biol Chem 278:37459–37464PubMedCrossRef
8.
Gaugler M, Squiban C, Claraz M, et al. (1998) Characterization of the response of human bone marrow endothelial cells to in vitro Irradiation. Br J Haematol 103:980–989CrossRef
9.
Harrington E, Smeglin A, Newton J, Ballard G, Rounds S (2001) Protein tyrosine phosphatase-dependent proteoiysis of focal adhesion complexes in endothelial cell apoptosis. Am J Physiol 280:L342–L353
10.
Hebbar M, Peyrat J (2000) Significance of soluble endothelial molecule E-selectin in patients with breast cancer. Int J Biol Markers 15:15–21PubMed
11.
Hooft van Huijsduijnen R, Whelan J, Pescini R, et al. (1992) A T-cell enhancer cooperates with NF-κB to yield cytokine induction of E-selectin gene transcription in endothelial cells. J Biol Chem 267;22385–22391
12.
Ilan N, Mohsenin A, Cheung L, Madri J (2001) PECAM-1 shedding during apoptosis generates a membrane-anchored truncated molecule with unique signaling characteristics. FASEB J 15:362–372PubMedCrossRef
13.
Janson C, Ludviksdottir D, Gunnbjornsdottir M, et al. (2005) Circulating adhesion molecules in allergic and non-allergic asthma. Respir Med 99:45–51PubMedCrossRef
14.
Joucher F, Mazmanian G, German-Fattal M (2005) Role of E-selectin in cell apoptosis induced by allogeneic blood perfusion in isolated mouse lung. Transplantation 80:666–672PubMedCrossRef
15.
Kayal S, Jais J, Aguini N, Chaudiere J, Labrousse J (1998) Elevated Circulating E-selectin, intercellular adhesion molecule 1, and von Willebrand factor in patients with severe infection. Am J Respir Crit Care Med 157:776–784PubMed
16.
Kern P, Keilholz L, Forster C, et al. (2000) UVB-irradiated T-cells undergoing apoptosis lose L-selectin by metalloprotese-mediated shedding. Int J Radiat Biol 76:1265–1271PubMedCrossRef
17.
Kerner T, Ahlers O, Reschreiter H, Mockel BCM, Gerlach H (2001) Adhesion molecules in differenct treatments of acute myocardial infarction. Crit Care 5:145–150PubMedCrossRef
18.
Koch A, Turkiewicz W, Harlow L, Pope R (1993) Soluble E-selectin in arthritis. Clin Immunol Immunopathol 69:29–35CrossRef
19.
Koch A, Halloran M, Haskeil C, Shah M, Polverini P (1995) Angiogenesis mediated by soluble forms of E-selectin and vascular cell adhesion molecule-I. Nature 376:517–519PubMedCrossRef
20.
Kramer K, Harrington E, Lu Q, et al. (2003) Isoprenylcysteine carboxyl methyltransferase activity modulates endothelial cell apoptosis. Mol Biol Cell 14:848–857PubMedCrossRef
21.
Kumar P, Hosaka S, Koch A (2001) Soluble E-selectin induces monocyte chemotaxis through Src family tyrosine kinases. J Biol Chem 276:21039–21045PubMedCrossRef
22.
Leeuwenberg J, Smeets E, Neefjes J, et al. (1992) E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77:543–549PubMed
23.
Lo S, Lee S, Ramos R, et al. (1991) Endothelial-leukocyte adhesion molecule 1 stimulates the adhesive activity of leukocyte integrin CR3 (CDllb/CDI8, Mac-I, αmβ2) on human neutrophils. J Exp Med 173: 1493–1500PubMedCrossRef
24.
Magro F, Araujo F, Pereira P, et al. (2004) Soluble selectins, sICAM, sVCAM, and angiogenic proteins in different activity groups of patients with inflammatory bowel disease. Dig Dis Sci 49:1265–1274PubMedCrossRef
25.
Misaghi S, Pacold M, Blom D, Ploegh H, Korbel G (2004) Using a small molecule inhibitor of peptide: N-glycanase to probe its role in glycoprotein turnover. Chem Biol 11:1677–1687PubMedCrossRef
26.
Misaghi S, Korbei G, Kessler B, Spooner E, Ploegh H (2006) z-VAD-fink inhibits peptide:N-glycanase and may result in ER stress. Cell Death Differ 13:163–165PubMedCrossRef
27.
Montefort S, Lai C, Kapahi P, et al. (1994) Circulating adhesion molecules in asthma. Am J Respir Crit Care Med 149:1149–1152PubMed
28.
Moss M, Gillespie M, Ackerson L, et al. (1996) Endothelial cell activity varies in patients at risk for the adult respiratory distress syndrome. Crit Care Med 24:1782–1786PubMedCrossRef
29.
Nozawa F, Hirota M, Okabe A, et al. (2000) Elastase activity enhances the adhesion of neutrophil and cancer cells to vascular endothelial cells. J Surg Res 94:153–158PubMedCrossRef
30.
Pahlsson P, Strindhall J, Srinivas U, Lundblad A (1995) Role of N-linked glycosylation in expression of E-selectin on human endothelial cells. Eur J Immunol 25:2452–2459PubMed
31.
Pigott R, Dillon L, Hemingway I, Gearing A (1992) Soluble forms of E-selectin, IC AM-1 and VCAM-1 are present in the supernatants of cytokine activated cultured endothelial cells. Biochem and Biophys Res Commun 187:584–589CrossRef
32.
Reinhart K, Bayer O, Brunkhorst F, Meisner M (2002) Markers of endothelial damage in organ dysfiinction and sepsis. Crit Care Med 30:S302–S312PubMedCrossRef
33.
Smith C (1997) Potential significance of circulating E-selectin. Circulation 95:1986–1988PubMed
34.
Stefanec T (2000) Endothelial apoptosis: could it have a role in the pathogenesis and treatment of disease? Chest 117:841–854PubMedCrossRef
35.
Subramaniam M, Koedam J, Wagner D (1993) Divergent fates of P- and E-selectins after their expression on the plasma membrane. Mol Biol Cell 4:791–801PubMed
36.
Tang R, Chen S, Soong W, Chung R (2002) Circulating adhesion molecules in sera of asthmatic children. Pediatric Pulmonology 33:249–254PubMedCrossRef
37.
von Asmuth E, Smeets E, Ginsei L, et al. (1992) Evidence for endocytosis of E-selectin in human endothelial cells. Eur J Immunol 22:2519–2526
Metadata
Title
Release of Soluble E-Selectin from Activated Endothelial Cells upon Apoptosis
Authors
Elizabeth O. Harrington
Tihomir Stefanec
Julie Newton
Sharon Rounds
Publication date
01-10-2006
Publisher
Springer-Verlag
Published in
Lung / Issue 5/2006
Print ISSN: 0341-2040
Electronic ISSN: 1432-1750
DOI
https://doi.org/10.1007/s00408-005-2589-5

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